Enhanced dereplication of fungal cultures via use of mass defect filtering.
J Antibiot (Tokyo)
; 70(5): 553-561, 2017 May.
Article
en En
| MEDLINE
| ID: mdl-28074050
ABSTRACT
Effective and rapid dereplication is a hallmark of present-day drug discovery from natural sources. This project strove to both decrease the time and expand the structural diversity associated with dereplication methodologies. A 5 min liquid chromatographic run time employing heated electrospray ionization (HESI) was evaluated to determine whether it could be used as a faster alternative over the 10 min ESI method we reported previously. Results revealed that the 5 min method was as sensitive as the 10 min method and, obviously, was twice as fast. To facilitate dereplication, the retention times, UV absorption maxima, full-scan HRMS and MS/MS were cross-referenced with an in-house database of over 300 fungal secondary metabolites. However, this strategy was dependent upon the makeup of the screening in-house database. Thus, mass defect filtering (MDF) was explored as an additional targeted screening strategy to permit identification of structurally related components. The use of a dereplication platform incorporating the 5 min chromatographic method together with MDF facilitated rapid and effective identification of known compounds and detection of structurally related analogs in extracts of fungal cultures.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Productos Biológicos
/
Cromatografía Liquida
/
Descubrimiento de Drogas
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Metabolismo Secundario
/
Hongos
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
J Antibiot (Tokyo)
Año:
2017
Tipo del documento:
Article
País de afiliación:
Estados Unidos