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Hyperforin Exhibits Antigenotoxic Activity on Human and Bacterial Cells.
Imreova, Petronela; Feruszova, Jana; Kyzek, Stanislav; Bodnarova, Kristina; Zduriencikova, Martina; Kozics, Katarina; Mucaji, Pavel; Galova, Eliska; Sevcovicova, Andrea; Miadokova, Eva; Chalupa, Ivan.
Afiliación
  • Imreova P; Department of Genetics, Faculty of Natural Sciences, Comenius University, Mlynská dolina B1, Ilkovicova 6, 842 15 Bratislava, Slovakia. petra.imreova@gmail.com.
  • Feruszova J; Department of Genetics, Faculty of Natural Sciences, Comenius University, Mlynská dolina B1, Ilkovicova 6, 842 15 Bratislava, Slovakia. jana.feruszova@gmail.com.
  • Kyzek S; Department of Genetics, Faculty of Natural Sciences, Comenius University, Mlynská dolina B1, Ilkovicova 6, 842 15 Bratislava, Slovakia. stanislavkyzek@gmail.com.
  • Bodnarova K; Department of Genetics, Faculty of Natural Sciences, Comenius University, Mlynská dolina B1, Ilkovicova 6, 842 15 Bratislava, Slovakia. bodnarova.kristina@gmail.com.
  • Zduriencikova M; Cancer Research Institute, Biomedical Research Center, SAS, Dúbravská cesta 9, 845 05 Bratislava, Slovakia. martina.zduriencikova@savba.sk.
  • Kozics K; Cancer Research Institute, Biomedical Research Center, SAS, Dúbravská cesta 9, 845 05 Bratislava, Slovakia. katarina.kozics@savba.sk.
  • Mucaji P; Department of Pharmacognosy and Botany, Faculty of Pharmacy, Comenius University, Odbojárov 10, 832 32 Bratislava, Slovakia. mucaji@fpharm.uniba.sk.
  • Galova E; Department of Genetics, Faculty of Natural Sciences, Comenius University, Mlynská dolina B1, Ilkovicova 6, 842 15 Bratislava, Slovakia. galova@fns.uniba.sk.
  • Sevcovicova A; Department of Genetics, Faculty of Natural Sciences, Comenius University, Mlynská dolina B1, Ilkovicova 6, 842 15 Bratislava, Slovakia. sevcovicova@fns.uniba.sk.
  • Miadokova E; Department of Genetics, Faculty of Natural Sciences, Comenius University, Mlynská dolina B1, Ilkovicova 6, 842 15 Bratislava, Slovakia. miadokova@fns.uniba.sk.
  • Chalupa I; Cancer Research Institute, Biomedical Research Center, SAS, Dúbravská cesta 9, 845 05 Bratislava, Slovakia. ivan.chalupa@savba.sk.
Molecules ; 22(1)2017 Jan 21.
Article en En | MEDLINE | ID: mdl-28117734
Hyperforin (HF), a substance that accumulates in the leaves and flowers of Hypericum perforatum L. (St. John's wort), consists of a phloroglucinol skeleton with lipophilic isoprene chains. HF exhibits several medicinal properties and is mainly used as an antidepressant. So far, the antigenotoxicity of HF has not been investigated at the level of primary genetic damage, gene mutations, and chromosome aberrations, simultaneously. The present work is designed to investigate the potential antigenotoxic effects of HF using three different experimental test systems. The antigenotoxic effect of HF leading to the decrease of primary/transient promutagenic genetic changes was detected by the alkaline comet assay on human lymphocytes. The HF antimutagenic effect leading to the reduction of gene mutations was assessed using the Ames test on the standard Salmonella typhimurium (TA97, TA98, and TA100) bacterial strains, and the anticlastogenic effect of HF leading to the reduction of chromosome aberrations was evaluated by the in vitro mammalian chromosome aberration test on the human tumor cell line HepG2 and the non-carcinogenic cell line VH10. Our findings provided evidence that HF showed antigenotoxic effects towards oxidative mutagen zeocin in the comet assay and diagnostic mutagen (4-nitroquinoline-1-oxide) in the Ames test. Moreover, HF exhibited an anticlastogenic effect towards benzo(a)pyrene and cisplatin in the chromosome aberration test.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Floroglucinol / Salmonella typhimurium / Terpenos / Bleomicina / Daño del ADN / Aberraciones Cromosómicas / Antimutagênicos Límite: Humans Idioma: En Revista: Molecules Asunto de la revista: BIOLOGIA Año: 2017 Tipo del documento: Article País de afiliación: Eslovaquia Pais de publicación: Suiza

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Floroglucinol / Salmonella typhimurium / Terpenos / Bleomicina / Daño del ADN / Aberraciones Cromosómicas / Antimutagênicos Límite: Humans Idioma: En Revista: Molecules Asunto de la revista: BIOLOGIA Año: 2017 Tipo del documento: Article País de afiliación: Eslovaquia Pais de publicación: Suiza