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Structural Insights into a Hemoglobin-Albumin Cluster in Aqueous Medium.
Shinohara, Ryuichi; Yamada, Taiga; Schade, Boris; Böttcher, Christoph; Sato, Takaaki; Sugimura, Natsuhiko; Shibue, Toshimichi; Komatsu, Teruyuki.
Afiliación
  • Shinohara R; Department of Applied Chemistry, Faculty of Science and Engineering, Chuo University , 1-13-27 Kasuga, Bunkyo-ku, Tokyo 112-8551, Japan.
  • Yamada T; Department of Applied Chemistry, Faculty of Science and Engineering, Chuo University , 1-13-27 Kasuga, Bunkyo-ku, Tokyo 112-8551, Japan.
  • Schade B; Research Center of Electron Microscopy, Institute of Chemistry and Biochemistry, Freie Universität Berlin , Fabeckstrasse 36a, 14195 Berlin, Germany.
  • Böttcher C; Research Center of Electron Microscopy, Institute of Chemistry and Biochemistry, Freie Universität Berlin , Fabeckstrasse 36a, 14195 Berlin, Germany.
  • Sato T; Department of Chemistry and Materials, Faculty of Textile Science and Technology, Shinshu University , 3-15-1 Tokida, Ueda-shi, Nagano 386-8567, Japan.
  • Sugimura N; Materials Characterization Central Laboratory, Waseda University , 3-4-1 Okubo, Shinjuku-ku, Tokyo 169-8555, Japan.
  • Shibue T; Materials Characterization Central Laboratory, Waseda University , 3-4-1 Okubo, Shinjuku-ku, Tokyo 169-8555, Japan.
  • Komatsu T; Department of Applied Chemistry, Faculty of Science and Engineering, Chuo University , 1-13-27 Kasuga, Bunkyo-ku, Tokyo 112-8551, Japan.
J Phys Chem Lett ; 8(4): 819-824, 2017 Feb 16.
Article en En | MEDLINE | ID: mdl-28151688
ABSTRACT
A hemoglobin (Hb) wrapped covalently by three human serum albumins (HSAs) is a triangular protein cluster designed as an artificial O2-carrier and red blood cell substitute. We report the structural insights into this Hb-HSA3 cluster in aqueous medium revealed by 3D reconstruction based on cryogenic transmission electron microscopy (cryo-TEM) data and small-angle X-ray scattering (SAXS) measurements. Cryo-TEM observations showed individual particles with approximately 15 nm diameter in the vitrified ice layer. Subsequent image processing and 3D reconstruction proved the expected spatial arrangements of an Hb in the center and three HSAs at the periphery. SAXS measurements demonstrated the monodispersity of the Hb-HSA3 cluster having a molecular mass of 270 kDa. The pair-distance distribution function suggested the existence of oblate-like particles with a maximum dimeter of ∼17 nm. The supramolecular 3D structure reconstructed from the SAXS intensity using an ab initio procedure was similar to that obtained from cryo-TEM data.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Hemoglobinas / Agua / Microscopía Electrónica de Transmisión / Albúmina Sérica Humana Límite: Humans Idioma: En Revista: J Phys Chem Lett Año: 2017 Tipo del documento: Article País de afiliación: Japón
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Hemoglobinas / Agua / Microscopía Electrónica de Transmisión / Albúmina Sérica Humana Límite: Humans Idioma: En Revista: J Phys Chem Lett Año: 2017 Tipo del documento: Article País de afiliación: Japón