Isolation and characterization of a novel pollen-specific promoter in maize (Zea mays L.).
Genome
; 60(6): 485-495, 2017 Jun.
Article
en En
| MEDLINE
| ID: mdl-28177828
ABSTRACT
ZmSTK2_USP, located on the long arm of chromosome 4, belongs to the serine/threonine kinase gene in maize. The sequence analysis of 2100 bp upstream from the start codon ATG has shown that it contains cis-element motifs and two types of anther/pollen-specific promoter elements (GTGA and AGAAA), suggesting that it is the pollen-specific promoter. To investigate the function of ZmSTK2_USP promoter, the GUS gene fusion system was employed. In proZmSTK2_USP-GUS genetically modified plants, GUS activity was detected in mature pollen grains and pollen tubes but not found in other floral and vegetative tissues. These results show that proZmSTK2_USP is the pollen-specific promoter and drives pollen-specific activity during the middle stage of pollen development until pollen maturation.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Polen
/
Regiones Promotoras Genéticas
/
Zea mays
Idioma:
En
Revista:
Genome
Asunto de la revista:
GENETICA
Año:
2017
Tipo del documento:
Article
País de afiliación:
China