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Microbial diversity of a full-scale UASB reactor applied to poultry slaughterhouse wastewater treatment: integration of 16S rRNA gene amplicon and shotgun metagenomic sequencing.
Delforno, Tiago Palladino; Lacerda Júnior, Gileno Vieira; Noronha, Melline F; Sakamoto, Isabel K; Varesche, Maria Bernadete A; Oliveira, Valéria M.
Afiliación
  • Delforno TP; Microbial Resources Division, Research Center for Chemistry, Biology and Agriculture (CPQBA), Campinas University - UNICAMP, Campinas, São Paulo, Brazil.
  • Lacerda Júnior GV; Microbial Resources Division, Research Center for Chemistry, Biology and Agriculture (CPQBA), Campinas University - UNICAMP, Campinas, São Paulo, Brazil.
  • Noronha MF; Microbial Resources Division, Research Center for Chemistry, Biology and Agriculture (CPQBA), Campinas University - UNICAMP, Campinas, São Paulo, Brazil.
  • Sakamoto IK; Laboratory of Biological Processes, Department of Hydraulics and Sanitation, Engineering School of São Carlos - University of São Paulo (EESC - USP) Campus II, São Carlos, São Paulo, Brazil.
  • Varesche MBA; Laboratory of Biological Processes, Department of Hydraulics and Sanitation, Engineering School of São Carlos - University of São Paulo (EESC - USP) Campus II, São Carlos, São Paulo, Brazil.
  • Oliveira VM; Microbial Resources Division, Research Center for Chemistry, Biology and Agriculture (CPQBA), Campinas University - UNICAMP, Campinas, São Paulo, Brazil.
Microbiologyopen ; 6(3)2017 06.
Article en En | MEDLINE | ID: mdl-28229558
The 16S rRNA gene amplicon and whole-genome shotgun metagenomic (WGSM) sequencing approaches were used to investigate wide-spectrum profiles of microbial composition and metabolic diversity from a full-scale UASB reactor applied to poultry slaughterhouse wastewater treatment. The data were generated by using MiSeq 2 × 250 bp and HiSeq 2 × 150 bp Illumina sequencing platforms for 16S amplicon and WGSM sequencing, respectively. Each approach revealed a distinct microbial community profile, with Pseudomonas and Psychrobacter as predominant genus for the WGSM dataset and Clostridium and Methanosaeta for the 16S rRNA gene amplicon dataset. The virome characterization revealed the presence of two viral families with Bacteria and Archaea as host, Myoviridae, and Siphoviridae. A wide functional diversity was found with predominance of genes involved in the metabolism of acetone, butanol, and ethanol synthesis; and one-carbon metabolism (e.g., methanogenesis). Genes related to the acetotrophic methanogenesis pathways were more abundant than methylotrophic and hydrogenotrophic, corroborating the taxonomic results that showed the prevalence of the acetotrophic genus Methanosaeta. Moreover, the dataset indicated a variety of metabolic genes involved in sulfur, nitrogen, iron, and phosphorus cycles, with many genera able to act in all cycles. BLAST analysis against Antibiotic Resistance Genes Database (ARDB) revealed that microbial community contained 43 different types of antibiotic resistance genes, some of them were associated with growth chicken promotion (e.g., bacitracin, tetracycline, and polymyxin).
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Mataderos / Purificación del Agua / Reactores Biológicos / Biota / Aguas Residuales Tipo de estudio: Risk_factors_studies Límite: Animals Idioma: En Revista: Microbiologyopen Año: 2017 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Reino Unido

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Mataderos / Purificación del Agua / Reactores Biológicos / Biota / Aguas Residuales Tipo de estudio: Risk_factors_studies Límite: Animals Idioma: En Revista: Microbiologyopen Año: 2017 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Reino Unido