Reacylation of platelet activating factor with eicosapentaenoic acid in fish-oil-enriched monkey neutrophils.
Biochim Biophys Acta
; 922(2): 214-20, 1987 Nov 21.
Article
en En
| MEDLINE
| ID: mdl-2823907
ABSTRACT
Platelet activating factor (PAF) is rapidly metabolized via a deacetylation reacylation pathway which shows striking specificity for arachidonate at the sn-2 position of the 1-O-alkyl-2-acyl-GPC thus formed. We have now examined the effects of a diet enriched in fish oils on the metabolism of PAF and specificity for arachidonate in the reacylation reaction. [3H]PAF was incubated for various lengths of time with neutrophils from monkeys fed a control diet or one enriched in fish oils. The [3H]PAF added to the cell suspension was rapidly converted to 1-O-alkyl-2-acyl-GPC. Reverse-phase HPLC analysis of the acyl chains added at the sn-2 position revealed that arachidonate was the major fatty acid incorporated into the 1-O-alkyl-2-acyl-GPC formed by neutrophils from monkeys on the control diet. In contrast, both 1-O-alkyl-2-arachidonoyl-GPC and 1-O-alkyl-2-eicosapentaenoyl-GPC were formed by the fish-oil-enriched neutrophils. We also report on the fatty acid composition of neutrophil phospholipids during such a diet.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Aceites de Pescado
/
Factor de Activación Plaquetaria
/
Ácido Eicosapentaenoico
/
Neutrófilos
Límite:
Animals
Idioma:
En
Revista:
Biochim Biophys Acta
Año:
1987
Tipo del documento:
Article