Hydrophobically modified polyethylenimine-based ternary complexes for targeting brain tumor: stability, in vitro and in vivo studies.
Artif Cells Nanomed Biotechnol
; 45(8): 1685-1698, 2017 Dec.
Article
en En
| MEDLINE
| ID: mdl-28278583
ABSTRACT
Hydrophobic modification of low molecular weight polyethylenimine (PEI 2 kDa) by cholic acid (ChA) was done to obtain PEI2-ChA. The nanoplexes of PEI2-ChA with gWIZ-GFP demonstrated increase transfection efficiency (â¼27%) in NT8e cell lines. The cell-cycle analysis of NT8e cells (p53 mutant) treated with transferrin containing nanoplexes showed increased apoptosis of cells. In vitro protein expression revealed expression of exogenous p53 protein. In vivo imaging of mice showed localized signal for GFP protein in brain region. The tumors of mice treated with transferrin containing nanoplexes of PEI2-ChA were â¼5 times smaller in size than the tumor of untreated animals.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Polietileneimina
/
Neoplasias Encefálicas
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Portadores de Fármacos
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Interacciones Hidrofóbicas e Hidrofílicas
Límite:
Animals
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Female
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Humans
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Male
Idioma:
En
Revista:
Artif Cells Nanomed Biotechnol
Año:
2017
Tipo del documento:
Article
País de afiliación:
India