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Proteomic profiling of fetal esophageal epithelium, esophageal cancer, and tumor-adjacent esophageal epithelium and immunohistochemical characterization of a representative differential protein, PRX6.
Guo, Jun-Hui; Xing, Guo-Lan; Fang, Xin-Hui; Wu, Hui-Fang; Zhang, Bo; Yu, Jin-Zhong; Fan, Zong-Min; Wang, Li-Dong.
Afiliación
  • Guo JH; Jun-Hui Guo, Henan Province Hospital of TCM, Zhengzhou 450000, Henan Province, China.
  • Xing GL; Jun-Hui Guo, Henan Province Hospital of TCM, Zhengzhou 450000, Henan Province, China.
  • Fang XH; Jun-Hui Guo, Henan Province Hospital of TCM, Zhengzhou 450000, Henan Province, China.
  • Wu HF; Jun-Hui Guo, Henan Province Hospital of TCM, Zhengzhou 450000, Henan Province, China.
  • Zhang B; Jun-Hui Guo, Henan Province Hospital of TCM, Zhengzhou 450000, Henan Province, China.
  • Yu JZ; Jun-Hui Guo, Henan Province Hospital of TCM, Zhengzhou 450000, Henan Province, China.
  • Fan ZM; Jun-Hui Guo, Henan Province Hospital of TCM, Zhengzhou 450000, Henan Province, China.
  • Wang LD; Jun-Hui Guo, Henan Province Hospital of TCM, Zhengzhou 450000, Henan Province, China.
World J Gastroenterol ; 23(8): 1434-1442, 2017 Feb 28.
Article en En | MEDLINE | ID: mdl-28293090
ABSTRACT

AIM:

To understand the molecular mechanism of esophageal cancer development and provide molecular markers for screening high-risk populations and early diagnosis.

METHODS:

Two-dimensional electrophoresis combined with mass spectrometry were adopted to screen differentially expressed proteins in nine cases of fetal esophageal epithelium, eight cases of esophageal cancer, and eight cases of tumor-adjacent normal esophageal epithelium collected from fetuses of different gestational age, or esophageal cancer patients from a high-risk area of esophageal cancer in China. Immunohistochemistry (avidin-biotin-horseradish peroxidase complex method) was used to detect the expression of peroxiredoxin (PRX)6 in 91 cases of esophageal cancer, tumor-adjacent normal esophageal tissue, basal cell hyperplasia, dysplasia, and carcinoma in situ, as well as 65 cases of esophageal epithelium from fetuses at a gestational age of 3-9 mo.

RESULTS:

After peptide mass fingerprint analysis and search of protein databases, 21 differential proteins were identified; some of which represent a protein isoform. Varying degrees of expression of PRX6 protein, which was localized mainly in the cytoplasm, were detected in adult and fetal normal esophageal tissues, precancerous lesions, and esophageal cancer. With the progression of esophageal lesions, PRX6 protein expression showed a declining trend (P < 0.05). In fetal epithelium from fetuses at gestational age 3-6 mo, PRX6 protein expression showed a declining trend with age (P < 0.05). PRX6 protein expression was significantly higher in well-differentiated esophageal cancer tissues than in poorly differentiated esophageal cancer tissues (P < 0.05).

CONCLUSION:

Development and progression of esophageal cancer result from interactions of genetic changes (accumulation or superposition). PRX6 protein is associated with fetal esophageal development and cancer differentiation.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias Esofágicas / Carcinoma de Células Escamosas / Epitelio / Esófago / Peroxiredoxina VI Tipo de estudio: Screening_studies Límite: Adult / Aged / Female / Humans / Infant / Male / Middle aged Idioma: En Revista: World J Gastroenterol Asunto de la revista: GASTROENTEROLOGIA Año: 2017 Tipo del documento: Article País de afiliación: China

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias Esofágicas / Carcinoma de Células Escamosas / Epitelio / Esófago / Peroxiredoxina VI Tipo de estudio: Screening_studies Límite: Adult / Aged / Female / Humans / Infant / Male / Middle aged Idioma: En Revista: World J Gastroenterol Asunto de la revista: GASTROENTEROLOGIA Año: 2017 Tipo del documento: Article País de afiliación: China