Stress influenced increase in phenolic content and radical scavenging capacity of Rhodotorula glutinis CCY 20-2-26.
3 Biotech
; 3(1): 53-60, 2013 Feb.
Article
en En
| MEDLINE
| ID: mdl-28324345
ABSTRACT
Rhodotorula glutinis CCY 20-2-26 when grown under controlled stress of either NaCl (1-5 %) or H2O2 (1-5 mM) on basal media exhibited a twofold increase in its total phenolic contents. The radical scavenging capacities (RSCs) as determined by ABTS test were found to be highest in 4 mM H2O2 (1.44 mM TEAC mg-1) and 4 % NaCl (1.13 mM TEAC mg-1) as compared to control samples (0.41 mM TEAC mg-1). Similarly, the RSCs as determined by DPPH test were also highest in 4 % NaCl (1.83 mM TEAC mg-1) and 4 mM H2O2 (1.78 mM TEAC mg-1) compared to control (0.48 TEAC mg-1). The relative RSCs from EPR spin-trapping assay for H2O2-stressed cultures were highest in 1 mM H2O2 (56.1 µM TEAC g-1) whereas in NaCl-stressed cultures it was highest in 5 % NaCl (44.6 µM TEAC g-1) as compared to control (30.9 µM TEAC g-1). Five phenolic compounds (gallic acid, benzoic acid, catechin, caffeic acid and ferulic acid) were detected for the first time in R. glutinis CCY 20-2-26.
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Colección:
01-internacional
Base de datos:
MEDLINE
Idioma:
En
Revista:
3 Biotech
Año:
2013
Tipo del documento:
Article
País de afiliación:
India