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Three-Dimensional Live Imaging of Filamentous Fungi with Light Sheet-Based Fluorescence Microscopy (LSFM).
Pampaloni, Francesco; Knuppertz, Laura; Hamann, Andrea; Osiewacz, Heinz D; Stelzer, Ernst H K.
Afiliación
  • Pampaloni F; Physical Biology Group, Buchmann Institute for Molecular Life Sciences (BMLS), Goethe Universität Frankfurt am Main, Max-von-Laue-Str. 15, D-60438, Frankfurt am Main, Germany. francesco.pampaloni@physikalischebiologie.de.
  • Knuppertz L; Institute of Molecular Biosciences and Cluster of Excellence Frankfurt Macromolecular Complexes, Department of Biosciences, Goethe Universität Frankfurt am Main, Max-von-Laue-Str. 9, 60438, Frankfurt am Main, Germany.
  • Hamann A; Institute of Molecular Biosciences and Cluster of Excellence Frankfurt Macromolecular Complexes, Department of Biosciences, Goethe Universität Frankfurt am Main, Max-von-Laue-Str. 9, 60438, Frankfurt am Main, Germany.
  • Osiewacz HD; Institute of Molecular Biosciences and Cluster of Excellence Frankfurt Macromolecular Complexes, Department of Biosciences, Goethe Universität Frankfurt am Main, Max-von-Laue-Str. 9, 60438, Frankfurt am Main, Germany.
  • Stelzer EHK; Physical Biology Group, Buchmann Institute for Molecular Life Sciences (BMLS), Goethe Universität Frankfurt am Main, Max-von-Laue-Str. 15, D-60438, Frankfurt am Main, Germany.
Methods Mol Biol ; 1563: 19-31, 2017.
Article en En | MEDLINE | ID: mdl-28324599
ABSTRACT
We describe a method for the three-dimensional live imaging of filamentous fungi with light sheet-based fluorescence microscopy (LSFM). LSFM provides completely new opportunities to investigate the biology of fungal cells and other microorganisms with high spatial and temporal resolution. As an example, we study the established aging model Podospora anserina. The protocol explains the mounting of the live fungi for the light sheet imaging, the imaging procedure and illustrates basic image processing of data.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Imagenología Tridimensional / Hongos / Microscopía Fluorescente Idioma: En Revista: Methods Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2017 Tipo del documento: Article País de afiliación: Alemania
Texto completo
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Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Imagenología Tridimensional / Hongos / Microscopía Fluorescente Idioma: En Revista: Methods Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2017 Tipo del documento: Article País de afiliación: Alemania