miR217 inhibits osteogenic differentiation of rat bone marrowderived mesenchymal stem cells by binding to Runx2.
Mol Med Rep
; 15(5): 3271-3277, 2017 May.
Article
en En
| MEDLINE
| ID: mdl-28339007
The elucidation of the underlying molecular mechanisms regulating the osteogenic differentiation of bone marrowderived mesenchymal stem cells (BMSCs) is of great importance in improving the treatment of boneassociated diseases. MicroRNAs (miRNAs) have been proven to regulate the osteogenic differentiation of BMSCs. The present study investigated the role of miR217 in the osteogenic differentiation of rat BMSCs. It was observed that miR217 expression levels were downregulated during the process of osteogenic differentiation. Subsequently, a dualluciferase reporter gene assay demonstrated that miR217 targets a putative binding site in the 3'untranslated region of the runt related transcription factor 2 (Runx2) gene, which is a key transcription factor for osteogenesis. It was then demonstrated that overexpression of miR217 attenuated the osteogenesis of BMSCs and downregulated the expression of Runx2, whereas inhibition of miR217 promoted osteoblastic differentiation and upregulated Runx2 expression. Furthermore, the extracellular signalregulated kinase (ERK) and p38 mitogenactivated protein kinase (p38 MAPK) signaling pathways were investigated during osteogenic induction, and the data indicated that miR217 may exert a negative effect on the osteogenic differentiation of BMSCs through alteration of ERK and p38 MAPK phosphorylation. The present study therefore concluded that miR217 functions as a negative regulator of BMSC osteogenic differentiation via the inhibition of Runx2 expression, and the underlying molecular mechanisms may partially be attributed to mediation by the ERK and p38 MAPK signaling pathways.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Diferenciación Celular
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MicroARNs
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Subunidad alfa 1 del Factor de Unión al Sitio Principal
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Células Madre Mesenquimatosas
Límite:
Animals
Idioma:
En
Revista:
Mol Med Rep
Año:
2017
Tipo del documento:
Article
Pais de publicación:
Grecia