In vivo and in vitro receptor autoradiography of the human brain using an 11C-labelled benzodiazepine analogue.
Neurosci Lett
; 85(3): 304-10, 1988 Mar 10.
Article
en En
| MEDLINE
| ID: mdl-2834674
ABSTRACT
In vitro autoradiography of an 11C-labelled ligand, Ro 15-1788, has been used to visualize benzodiazepine binding sites in large human brain cryo-sections. In parallel, using the same radioligand, an in vivo study of a human healthy volunteer was performed, by means of positron emission tomography (PET). The in vitro and in vivo mapping of the ligand demonstrated a very similar binding pattern, although the poor resolution of PET precluded a full discrimination of fine details. The 11C-autoradiograms showed good spatial resolution, even with distinction of different layers in the cerebral and cerebellar cortex. In a separate experiment, the spatial resolution of 11C-autoradiography was found to be 180 microns, using 80-microns-thick cryo-sections. It is emphasized that in vitro and in vivo studies of the same radioligand give complementary information, which is valuable in the assessment of PET images.
Buscar en Google
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Encéfalo
/
Receptores de GABA-A
Límite:
Aged
/
Humans
/
Male
Idioma:
En
Revista:
Neurosci Lett
Año:
1988
Tipo del documento:
Article
País de afiliación:
Suecia