Isolation and characterization of alphoid DNA sequences specific for the pericentric regions of chromosomes 4, 5, 9, and 19.
Cytogenet Cell Genet
; 47(3): 144-8, 1988.
Article
en En
| MEDLINE
| ID: mdl-2837365
ABSTRACT
We have cloned and characterized two distinct types of alphoid DNA elements. Probe pG-Xba 11/340 was obtained by random cloning of human satellite DNA and contains two basic units with overall 88% homology to the 171-bp consensus alphoid sequence. pG-Xba 11/340-like elements are represented about 2,000-4,000 times in the haploid genome and, by in situ hybridization, are found exclusively at the primary constrictions of chromosomes 4 and 9. Probe pG-A16 was cloned from a chromosome 19-specific cosmid library and represents a 2.25-kb higher-order DNA element which is present at roughly 75-150 copies per haploid genome and which hybridizes to the centromeres of chromosomes 5 and 19. Using the pG-A16 probe, further genetic and physical dissection of the central area of chromosome 19 can be envisaged.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Cromosomas Humanos Par 9
/
Cromosomas Humanos Par 19
/
ADN Satélite
/
Secuencia de Bases
/
Homología de Secuencia de Ácido Nucleico
/
Cromosomas Humanos 4-5
Límite:
Humans
Idioma:
En
Revista:
Cytogenet Cell Genet
Año:
1988
Tipo del documento:
Article
País de afiliación:
Países Bajos