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Detection and differentiation of Entamoeba histolytica and Entamoeba dispar in clinical samples through PCR-denaturing gradient gel electrophoresis.
López-López, P; Martínez-López, M C; Boldo-León, X M; Hernández-Díaz, Y; González-Castro, T B; Tovilla-Zárate, C A; Luna-Arias, J P.
Afiliación
  • López-López P; Family Medicine Unit 43, Social Security Mexican Institute, Cardenas, Tabasco, Mexico.
  • Martínez-López MC; Health Sciences Academic Division, Juarez Autonomous University of Tabasco, Villahermosa, Tabasco, Mexico.
  • Boldo-León XM; Health Sciences Academic Division, Juarez Autonomous University of Tabasco, Villahermosa, Tabasco, Mexico.
  • Hernández-Díaz Y; Multidisciplinary Academic Division at Jalpa de Mendez, Juarez Autonomous University of Tabasco, Jalpa de Mendez, Tabasco, Mexico.
  • González-Castro TB; Multidisciplinary Academic Division at Jalpa de Mendez, Juarez Autonomous University of Tabasco, Jalpa de Mendez, Tabasco, Mexico.
  • Tovilla-Zárate CA; Multidisciplinary Academic Division at Comalcalco, Juarez Autonomous University of Tabasco, Comalcalco, Tabasco, Mexico.
  • Luna-Arias JP; Center for Research and Advanced Studies, National Polytechnic Institute, Mexico City, Mexico.
Braz J Med Biol Res ; 50(4): e5997, 2017 Apr 03.
Article en En | MEDLINE | ID: mdl-28380216
Amebiasis is one of the twenty major causes of disease in Mexico; however, the diagnosis is difficult due to limitations of conventional microscopy-based techniques. In this study, we analyzed stool samples using polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) to differentiate between Entamoeba histolytica (pathogenic) and E. dispar (non-pathogenic). The target for the PCR amplification was a small region (228 bp) of the adh112 gene selected to increase the sensitivity of the test. The study involved 62 stool samples that were collected from individuals with complaints of gastrointestinal discomfort. Of the 62 samples, 10 (16.1%) were positive for E. histolytica while 52 (83.9%) were negative. No sample was positive for E. dispar. These results were validated by nested PCR-RFLP (restriction fragment length polymorphism) and suggest that PCR-DGGE is a promising tool to differentiate among Entamoeba infections, contributing to determine the specific treatment for patients infected with E. histolytica, and therefore, avoiding unnecessary treatment of patients infected with the non-pathogenic E. dispar.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Reacción en Cadena de la Polimerasa / Entamoeba / Entamoeba histolytica / Electroforesis en Gel de Gradiente Desnaturalizante Tipo de estudio: Diagnostic_studies / Evaluation_studies Límite: Humans Idioma: En Revista: Braz J Med Biol Res Año: 2017 Tipo del documento: Article País de afiliación: México Pais de publicación: Brasil

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Reacción en Cadena de la Polimerasa / Entamoeba / Entamoeba histolytica / Electroforesis en Gel de Gradiente Desnaturalizante Tipo de estudio: Diagnostic_studies / Evaluation_studies Límite: Humans Idioma: En Revista: Braz J Med Biol Res Año: 2017 Tipo del documento: Article País de afiliación: México Pais de publicación: Brasil