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Generation of human iPSCs from urine derived cells of a non-affected control subject.
Afzal, Muhammad Zeeshan; Gartz, Melanie; Klyachko, Ekaterina A; Khan, Sadiya Sana; Shah, Sanjiv J; Gupta, Sweta; Shapiro, Amy D; Vaughan, Douglas E; Strande, Jennifer L.
Afiliación
  • Afzal MZ; Department of Medicine and Cardiovascular Center, Medical College of Wisconsin, Milwaukee, WI, United States.
  • Gartz M; Department of Medicine and Cardiovascular Center, Medical College of Wisconsin, Milwaukee, WI, United States.
  • Klyachko EA; Department of Medicine, Northwestern University-Feinberg School of Medicine, Chicago, IL, United States.
  • Khan SS; Department of Medicine, Northwestern University-Feinberg School of Medicine, Chicago, IL, United States.
  • Shah SJ; Department of Medicine, Northwestern University-Feinberg School of Medicine, Chicago, IL, United States.
  • Gupta S; Indiana Hemophilia and Thrombosis Center, Indianapolis, IN, United States.
  • Shapiro AD; Indiana Hemophilia and Thrombosis Center, Indianapolis, IN, United States.
  • Vaughan DE; Department of Medicine, Northwestern University-Feinberg School of Medicine, Chicago, IL, United States.
  • Strande JL; Department of Medicine and Cardiovascular Center, Medical College of Wisconsin, Milwaukee, WI, United States.
Stem Cell Res ; 18: 33-36, 2017 01.
Article en En | MEDLINE | ID: mdl-28395799
We have generated a human induced pluripotent stem cell (iPSC) line under feeder-free culture conditions using the urine derived cells (UCs) collected from non-affected control subjects to use as a comparison group for the iPSC lines containing a Plasminogen Activator Inhibitor-1 (PAI-1 homozygous/heterozygous) mutation. The Sendai Virus (SeV) vector encoding pluripotent Yamanaka transcription factors was used at a low multiplicity of infection to reprogram the UCs.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Orina / Reprogramación Celular / Células Madre Pluripotentes Inducidas Límite: Female / Humans Idioma: En Revista: Stem Cell Res Año: 2017 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido
Texto completo
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Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Orina / Reprogramación Celular / Células Madre Pluripotentes Inducidas Límite: Female / Humans Idioma: En Revista: Stem Cell Res Año: 2017 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido