CRISPR-Cas9 strategy for activation of silent Streptomyces biosynthetic gene clusters.
Nat Chem Biol
; 2017 Apr 10.
Article
en En
| MEDLINE
| ID: mdl-28398287
ABSTRACT
Here we report an efficient CRISPR-Cas9 knock-in strategy to activate silent biosynthetic gene clusters (BGCs) in streptomycetes. We applied this one-step strategy to activate multiple BGCs of different classes in five Streptomyces species and triggered the production of unique metabolites, including a novel pentangular type II polyketide in Streptomyces viridochromogenes. This potentially scalable strategy complements existing activation approaches and facilitates discovery efforts to uncover new compounds with interesting bioactivities.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Idioma:
En
Revista:
Nat Chem Biol
Asunto de la revista:
BIOLOGIA
/
QUIMICA
Año:
2017
Tipo del documento:
Article
País de afiliación:
Singapur