Molecular strategies to increase the levels of heterologous transcripts in Komagataella phaffii for protein production.
Bioengineered
; 8(5): 441-445, 2017 Sep 03.
Article
en En
| MEDLINE
| ID: mdl-28399696
ABSTRACT
Komagataella phaffii (formerly Pichia pastoris) is a well-known fungal system for heterologous protein production in the context of modern biotechnology. To obtain higher protein titers in this system many researchers have sought to optimize gene expression by increasing the levels of transcription of the heterologous gene. This has been typically achieved by manipulating promoter sequences or by generating clones bearing multiple copies of the desired gene. The aim of this work is to describe how these different molecular strategies have been applied in K. phaffii presenting their advantages and drawbacks.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Ascomicetos
/
Factores de Transcripción
/
Proteínas Recombinantes
/
Ingeniería de Proteínas
/
Regiones Promotoras Genéticas
/
Mejoramiento Genético
Idioma:
En
Revista:
Bioengineered
Año:
2017
Tipo del documento:
Article
País de afiliación:
Brasil