Design and synthesis of reduced graphene oxide based supramolecular scaffold: A benign microbial resistant network for enzyme immobilization and cell growth.
Mater Sci Eng C Mater Biol Appl
; 75: 1168-1177, 2017 Jun 01.
Article
en En
| MEDLINE
| ID: mdl-28415403
ABSTRACT
The present study describes the synthesis, characterization and biological application of reduced graphene oxide - chitosan (GC) based benign supramolecular scaffold (SMS). Various spectroscopic and microscopic analyses established the supramolecular interaction in between rGO and chitosan. The active performance of the developed material towards microbial resistivity, in vitro cell growth and as a scaffold for enzyme immobilizing matrix illustrates its unique implementation. Immobilization of polyphenol oxidase (PPO) onto GC lowers the Michaelis- Menten constant (Km) value and facilitates to achieve maximum velocity at low substrate concentration. Importantly GC shows no noteworthy cytotoxicity towards Wistar rat macrophage cells. Moreover, incorporation of gold nanoparticle further strengthens the microbial resistance properties of GC as well as improves its biocompatibility by reducing cytotoxicity. Therefore these unique features may inspire it to appear in large scale for industrial utilization.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Bacillus subtilis
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Monofenol Monooxigenasa
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Enzimas Inmovilizadas
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Escherichia coli
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Nanopartículas del Metal
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Oro
/
Grafito
Límite:
Animals
Idioma:
En
Revista:
Mater Sci Eng C Mater Biol Appl
Año:
2017
Tipo del documento:
Article
País de afiliación:
India