Two distinct periplasmic enzymes are responsible for tellurite/tellurate and selenite reduction by strain ER-Te-48 associated with the deep sea hydrothermal vent tube worms at the Juan de Fuca Ridge black smokers.
Arch Microbiol
; 199(8): 1113-1120, 2017 Oct.
Article
en En
| MEDLINE
| ID: mdl-28432382
ABSTRACT
Strain ER-Te-48 isolated from a deep-ocean hydrothermal vent tube worm is capable of resisting and reducing extremely high levels of tellurite, tellurate, and selenite, which are used for respiration anaerobically. Tellurite and tellurate reduction is accomplished by a periplasmic enzyme of 215 kDa comprised of 3 subunits (74, 42, and 25 kDa) in a 211 ratio. The optimum pH and temperature for activity is 8.0 and 35 °C, respectively. Tellurite reduction has a V max of 5.6 µmol/min/mg protein and a K m of 3.9 mM. In the case of the tellurate reaction, V max and K m were 2.6 µmol/min/mg protein and 2.6 mM, respectively. Selenite reduction is carried out by another periplasmic enzyme with a V max of 2.8 µmol/min/mg protein, K m of 12.1 mM, and maximal activity at pH 6.0 and 38 °C. This protein is 165 kDa and comprised of 3 subunits of 98, 44, and 23 kDa in a 111 ratio.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Telurio
/
Periplasma
/
Shewanella
/
Ácido Selenioso
/
Respiraderos Hidrotermales
Tipo de estudio:
Risk_factors_studies
Idioma:
En
Revista:
Arch Microbiol
Año:
2017
Tipo del documento:
Article
País de afiliación:
Canadá