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Regulation of uridine diphosphate-glucuronosyltransferase 2B15 expression during ovulation in the rat.
Dam, Phuong T M; Jang, You-Jee; Park, Jae-Il; Choi, Bum-Chae; Song, Sang-Jin; Chun, Sang-Young.
Afiliación
  • Dam PTM; School of Biological Sciences and Technology, Faculty of Life Science, Chonnam National University, Gwangju 500-757, Republic of Korea.
  • Jang YJ; School of Biological Sciences and Technology, Faculty of Life Science, Chonnam National University, Gwangju 500-757, Republic of Korea.
  • Park JI; Animal Facility of Aging Science, Korea Basic Science Institute, Gwangju 61186, Republic of Korea.
  • Choi BC; Center for Recurrent Miscarriage and Infertility, Creation and Love Women's Hospital, Gwangju 61917, Republic of Korea.
  • Song SJ; Center for Recurrent Miscarriage and Infertility, Creation and Love Women's Hospital, Gwangju 61917, Republic of Korea.
  • Chun SY; School of Biological Sciences and Technology, Faculty of Life Science, Chonnam National University, Gwangju 500-757, Republic of Korea.
Endocr J ; 64(6): 605-612, 2017 Jun 29.
Article en En | MEDLINE | ID: mdl-28442641
Uridine diphosphate-glucuronosyltransferase 2B15 (UGT2B15) conjugates 5α-androstane-3α, 17ß-diol (3α-diol) to 3α-diol glucuronide (3α-diol G) in steroid target tissues. The present study investigated the regulation of UGT2B15 expression during the ovulatory process in the rat. Real-time PCR analysis revealed that treatment of immature rats with equine chorionic gonadotropin followed by human chorionic gonadotropin transiently stimulated UGT2B15 gene expression in granulosa cells of preovulatory follicles within 6 h. The progesterone receptor antagonist RU486 suppressed the gonadotropin-induced UGT2B15 expression. The expression of UGT2B15 and the levels of 3α-diol G were transiently increased by luteinizing hormone (LH) treatment in cultured preovulatory follicles. The LH-stimulated UGT2B15 mRNA level in cultured preovulatory follicles was inhibited by inhibitors of adenylyl cyclase, phosphoinositide 3-kinase and mitogen-activated protein kinase. Furthermore, a vitamin D receptor agonist (calcitriol) suppressed the LH-stimulated UGT2B15 expression in a dose-dependent manner. Taken together, these results indicate that gonadotropins transiently stimulate UGT2B15 expression and activity in preovulatory follicles, and UGT2B15 mRNA levels are regulated by the progesterone receptor and vitamin D receptor.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ovulación / Transducción de Señal / Receptores de Progesterona / Glucuronosiltransferasa / Gonadotropinas / Células de la Granulosa Límite: Animals Idioma: En Revista: Endocr J Asunto de la revista: ENDOCRINOLOGIA Año: 2017 Tipo del documento: Article Pais de publicación: Japón

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ovulación / Transducción de Señal / Receptores de Progesterona / Glucuronosiltransferasa / Gonadotropinas / Células de la Granulosa Límite: Animals Idioma: En Revista: Endocr J Asunto de la revista: ENDOCRINOLOGIA Año: 2017 Tipo del documento: Article Pais de publicación: Japón