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TCDD influences reservoir of antibiotic resistance genes in murine gut microbiome.
Stedtfeld, Robert D; Stedtfeld, Tiffany M; Fader, Kelly A; Williams, Maggie R; Bhaduri, Prianca; Quensen, John; Zacharewski, Timothy R; Tiedje, James M; Hashsham, Syed A.
Afiliación
  • Stedtfeld RD; Department of Civil and Environmental Engineering, East Lansing, MI 48824, USA.
  • Stedtfeld TM; Department of Civil and Environmental Engineering, East Lansing, MI 48824, USA.
  • Fader KA; Institute for Integrative Toxicology, Michigan State University, East Lansing, MI 48824, USA.
  • Williams MR; Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, MI 48824, USA.
  • Bhaduri P; Department of Civil and Environmental Engineering, East Lansing, MI 48824, USA.
  • Quensen J; Department of Civil and Environmental Engineering, East Lansing, MI 48824, USA.
  • Zacharewski TR; Center for Microbial Ecology, Michigan State University, East Lansing, MI 48824, USA.
  • Tiedje JM; Institute for Integrative Toxicology, Michigan State University, East Lansing, MI 48824, USA.
  • Hashsham SA; Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, MI 48824, USA.
FEMS Microbiol Ecol ; 93(5)2017 05 01.
Article en En | MEDLINE | ID: mdl-28475713
Dysbiosis of the gut microbiome via antibiotics, changes in diet and infection can select for bacterial groups that more frequently harbor antimicrobial resistance genes (ARGs) and mobile genetic elements (MGEs). However, the impact of environmental toxicants on the reservoir of ARGs in the gut microbiome has received less attention. 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) is a potent aryl hydrocarbon receptor (AhR) agonist with multiple toxic health effects including immune dysfunction. The selective pressure of TCDD on the abundance of ARG and MGE-harboring gut populations was examined using C57BL/6 mice exposed to 0-30 µg/kg TCDD for 28 and 92 days with the latter having a 30-day recovery period. DNA extracted from temporally collected fecal pellets was characterized using a qPCR array with 384 assays targeting ARGs and MGEs. Fourteen genes, typically observed in Enterobacteriaceae, increased significantly within 8 days of initial dosing, persisted throughout the treatment period, and remained induced 30 days post dosing. A qPCR primer set targeting Enterobacteriaceae also showed 10- to 100-fold higher abundance in TCDD-treated groups, which was further verified using metagenomics. Results show a bloom of ARG-harboring bacterial groups in the gut due to a xenobiotic compound that is not a metal, biocide or antimicrobial.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Farmacorresistencia Bacteriana / Enterobacteriaceae / Disbiosis / Microbioma Gastrointestinal / Dibenzodioxinas Policloradas Límite: Animals Idioma: En Revista: FEMS Microbiol Ecol Año: 2017 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Farmacorresistencia Bacteriana / Enterobacteriaceae / Disbiosis / Microbioma Gastrointestinal / Dibenzodioxinas Policloradas Límite: Animals Idioma: En Revista: FEMS Microbiol Ecol Año: 2017 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido