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Development of a progesterone immunosensor based on thionine-graphene oxide composites platforms: Improvement by biotin-streptavidin-amplified system.
Dong, Xiu-Xiu; Yuan, Li-Peng; Liu, Yi-Xin; Wu, Min-Fu; Liu, Bo; Sun, Yuan-Ming; Shen, Yu-Dong; Xu, Zhen-Lin.
Afiliación
  • Dong XX; Guangdong Provincial Key Laboratory of Food Quality and Safety, South China Agricultural University, Guangzhou 510642, China; State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China.
  • Yuan LP; Guangdong Agriculture Industry Business Polytechnic College, Guangzhou 510507, China.
  • Liu YX; Guangdong Provincial Key Laboratory of Food Quality and Safety, South China Agricultural University, Guangzhou 510642, China.
  • Wu MF; Guangdong Provincial Key Laboratory of Food Quality and Safety, South China Agricultural University, Guangzhou 510642, China.
  • Liu B; Guangdong Agriculture Industry Business Polytechnic College, Guangzhou 510507, China.
  • Sun YM; Guangdong Provincial Key Laboratory of Food Quality and Safety, South China Agricultural University, Guangzhou 510642, China.
  • Shen YD; Guangdong Provincial Key Laboratory of Food Quality and Safety, South China Agricultural University, Guangzhou 510642, China.
  • Xu ZL; Guangdong Provincial Key Laboratory of Food Quality and Safety, South China Agricultural University, Guangzhou 510642, China. Electronic address: jallent@163.com.
Talanta ; 170: 502-508, 2017 Aug 01.
Article en En | MEDLINE | ID: mdl-28501203
Progesterone (P4) is a kind of hormone that can cause neuropathic disturbances in humans when the concentration overpasses a certain degree. In this work, an electrochemical immunosensor capable of detecting P4 sensitively and selectively was developed. Thionine-graphene oxide (Thi-GO) composites with excellent biocompatibility were synthesized and coated to a clear glassy carbon electrode. P4 coating antigen (P4-OVA) was immobilized to the electrode, then sample as well as biotinylated antibody (biotin-P4 Ab) were added. The free P4 can compete with P4-OVA for binding to biotin-P4 Ab. After the further addition of streptavidin-HRP, H2O2 was introduced to develop electrical signal for quantitative determination of P4. After careful optimization of assay conditions, the proposed immunosensor showed a linear range from 0.02 to 20ngmL-1 for P4 in milk samples. The averaged recoveries from spiked samples ranged from 84.0% to 102.0%, which correlated well with standard HPLC-MS/MS. The biosensor also showed good specificity, reproducibility and stability, indicating its potential application in monitoring of P4 in a simple and low cost manner.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fenotiazinas / Progesterona / Biotina / Técnicas Biosensibles / Estreptavidina / Leche / Grafito Tipo de estudio: Evaluation_studies Límite: Animals Idioma: En Revista: Talanta Año: 2017 Tipo del documento: Article País de afiliación: China Pais de publicación: Países Bajos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fenotiazinas / Progesterona / Biotina / Técnicas Biosensibles / Estreptavidina / Leche / Grafito Tipo de estudio: Evaluation_studies Límite: Animals Idioma: En Revista: Talanta Año: 2017 Tipo del documento: Article País de afiliación: China Pais de publicación: Países Bajos