Laser Desorption/Ionisation Mass Spectrometry Imaging of European Yew (Taxus baccata) on Gold Nanoparticle-enhanced Target.
Phytochem Anal
; 28(5): 448-453, 2017 Sep.
Article
en En
| MEDLINE
| ID: mdl-28523824
ABSTRACT
INTRODUCTION:
European yew (Taxus baccata) is a plant known to man for centuries as it produces many interesting and important metabolites. These chemical compounds were repeatedly analysed by various analytical techniques, but none of the methods used so far allowed the localisation of the chemical compounds within the tissue and also correlation between plant morphology and its biochemistry.OBJECTIVE:
Visualisation of the spatial distribution of yew metabolites with nanoparticle-based mass spectrometry imaging.METHODOLOGY:
Compounds occurring on cross-section of a one-year yew sprig has been transferred to gold nanoparticle-enhanced target (AuNPET) by imprinting. The imprint was then subjected to mass spectrometry imaging analysis.RESULTS:
Nanoparticle-enhanced mass spectrometry imaging made it possible to study the distribution of selected compounds in the European yew tissue, including taxanes - terpene alkaloids characteristic for the Taxus genus. Results prove that aspartate, taxinine M, baccatin IV and taxine B are located mainly in the cortex. Taxuspine W was located in the vascular tissue. Maleate was found to be located mainly in the phloem tissue. In contrast, the proton adduct of chlorophyll b was found in the external layer of twigs.CONCLUSION:
The results presented a high correlation between the location of compounds and the morphology of the plant, thus giving the opportunity to see the selected details of chemical structure of the analysed tissue for the first time. Copyright © 2017 John Wiley & Sons, Ltd.Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Extractos Vegetales
/
Taxus
/
Taxoides
/
Nanopartículas
Idioma:
En
Revista:
Phytochem Anal
Asunto de la revista:
BOTANICA
/
QUIMICA
Año:
2017
Tipo del documento:
Article
País de afiliación:
Polonia