Enzymatic Engineering of Live Bacterial Cell Surfaces Using Butelaseâ
1.
Angew Chem Int Ed Engl
; 56(27): 7822-7825, 2017 06 26.
Article
en En
| MEDLINE
| ID: mdl-28524544
ABSTRACT
Butelase-mediated ligation (BML) can be used to modify live bacterial cell surfaces with diverse cargo molecules. Surface-displayed butelase recognition motif NHV was first introduced at the C-terminal end of the anchoring protein OmpA on E.â
coli cells. This then served as a handle of BML for the functionalization of E.â
coli cell surfaces with fluorescein and biotin tags, a tumor-associated monoglycosylated peptide, and mCherry protein. The cell-surface ligation reaction was achieved at low concentrations of butelase and the labeling substrates. Furthermore, the fluorescein-labeled bacterial cells were used to show the interactions with cultured HeLa cells and with macrophages in live transgenic zebrafish, capturing the latter's powerful phagocytic effect in action. Together these results highlight the usefulness of butelaseâ
1 in live bacterial cell surface engineering for novel applications.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Proteínas de Plantas
/
Glicopéptidos
/
Escherichia coli
/
Ligasas
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Angew Chem Int Ed Engl
Año:
2017
Tipo del documento:
Article
País de afiliación:
Singapur