An in-vitro urinary catheterization model that approximates clinical conditions for evaluation of innovations to prevent catheter-associated urinary tract infections.
J Hosp Infect
; 97(1): 66-73, 2017 Sep.
Article
en En
| MEDLINE
| ID: mdl-28526271
ABSTRACT
BACKGROUND:
Catheter-associated urinary tract infections (CAUTI) account for approximately 25% of nosocomial infections globally, and often result in increased morbidity and healthcare costs. An additional concern is the presence of microbial biofilms which are major reservoirs of bacteria, especially antibiotic-resistant bacteria, in catheters. Since introduction of the use of closed drainage systems, innovations to combat CAUTI have not led to significant improvements in clinical outcomes. The lack of a robust laboratory platform to test new CAUTI preventive strategies may impede development of novel technologies.AIM:
To establish an in-vitro catheterization model (IVCM) for testing of technological innovations to prevent CAUTI.METHODS:
The IVCM consists of a continuous supply of urine medium flowing into a receptacle (bladder) where the urine is drained through a urinary catheter connected to an effluent collection vessel (drainage bag). Test organism(s) can be introduced conveniently into the bladder via a rubber septa port. Development of bacteriuria and microbial biofilm on the catheter can be determined subsequently.FINDINGS:
With an initial inoculum of Escherichia coli [â¼5×105 colony-forming units (cfu)/mL] into the bladder, a 100% silicone catheter and a commercially available silver-hydrogel catheter showed heavy biofilm colonization (â¼108 cfu/cm and â¼107 cfu/cm, respectively) with similar bacterial populations in the urine (bacteriuria) (â¼108 cfu/mL and â¼107 cfu/mL, respectively) within three days. Interestingly, an antimicrobial peptide (CP11-6A)-coated catheter showed negligible biofilm colonization and no detectable bacteriuria.CONCLUSION:
The IVCM is a useful preclinical approach to evaluate new strategies for the prevention of CAUTI.Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Infecciones Urinarias
/
Cateterismo Urinario
/
Infecciones por Escherichia coli
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Infecciones Relacionadas con Catéteres
/
Modelos Teóricos
Tipo de estudio:
Prognostic_studies
/
Risk_factors_studies
Límite:
Humans
/
Male
Idioma:
En
Revista:
J Hosp Infect
Año:
2017
Tipo del documento:
Article