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Microfluidic Capillary Electrophoresis-Mass Spectrometry for Analysis of Monosaccharides, Oligosaccharides, and Glycopeptides.
Khatri, Kshitij; Klein, Joshua A; Haserick, John R; Leon, Deborah R; Costello, Catherine E; McComb, Mark E; Zaia, Joseph.
Afiliación
  • Khatri K; Department of Biochemistry, Center for Biomedical Mass Spectrometry and ‡Bioinformatics Program, Boston University , Boston, Massachusetts 02215, United States.
  • Klein JA; Department of Biochemistry, Center for Biomedical Mass Spectrometry and ‡Bioinformatics Program, Boston University , Boston, Massachusetts 02215, United States.
  • Haserick JR; Department of Biochemistry, Center for Biomedical Mass Spectrometry and ‡Bioinformatics Program, Boston University , Boston, Massachusetts 02215, United States.
  • Leon DR; Department of Biochemistry, Center for Biomedical Mass Spectrometry and ‡Bioinformatics Program, Boston University , Boston, Massachusetts 02215, United States.
  • Costello CE; Department of Biochemistry, Center for Biomedical Mass Spectrometry and ‡Bioinformatics Program, Boston University , Boston, Massachusetts 02215, United States.
  • McComb ME; Department of Biochemistry, Center for Biomedical Mass Spectrometry and ‡Bioinformatics Program, Boston University , Boston, Massachusetts 02215, United States.
  • Zaia J; Department of Biochemistry, Center for Biomedical Mass Spectrometry and ‡Bioinformatics Program, Boston University , Boston, Massachusetts 02215, United States.
Anal Chem ; 89(12): 6645-6655, 2017 06 20.
Article en En | MEDLINE | ID: mdl-28530388
ABSTRACT
Glycomics and glycoproteomics analyses by mass spectrometry require efficient front-end separation methods to enable deep characterization of heterogeneous glycoform populations. Chromatography methods are generally limited in their ability to resolve glycoforms using mobile phases that are compatible with online liquid chromatography-mass spectrometry (LC-MS). The adoption of capillary electrophoresis-mass spectrometry methods (CE-MS) for glycomics and glycoproteomics is limited by the lack of convenient interfaces for coupling the CE devices to mass spectrometers. Here, we describe the application of a microfluidics-based CE-MS system for analysis of released glycans, glycopeptides and monosaccharides. We demonstrate a single CE method for three different modalities, thus contributing to comprehensive glycoproteomics analyses. In addition, we explored compatible sample derivatization methods. We used glycan TMT-labeling to improve electrophoretic migration and enable multiplexed quantitation by tandem MS. We used sialic acid linkage-specific derivatization methods to improve separation and the level of information obtained from a single analytical step. Capillary electrophoresis greatly improved glycoform separation for both released glycans and glycopeptides over that reported for chromatography modes more frequently employed for such analyses. Overall, the CE-MS method described here enables rapid setup and analysis of glycans and glycopeptides using mass spectrometry.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Oligosacáridos / Glicopéptidos / Técnicas Analíticas Microfluídicas / Monosacáridos Idioma: En Revista: Anal Chem Año: 2017 Tipo del documento: Article País de afiliación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Oligosacáridos / Glicopéptidos / Técnicas Analíticas Microfluídicas / Monosacáridos Idioma: En Revista: Anal Chem Año: 2017 Tipo del documento: Article País de afiliación: Estados Unidos