Differentiation of nestinnegative human hair follicle outer root sheath cells into neurons in vitro.
Mol Med Rep
; 16(1): 95-100, 2017 Jul.
Article
en En
| MEDLINE
| ID: mdl-28534946
ABSTRACT
A specialized quiescent population of hair follicle stem cells, residing in the hair follicle outer root sheath cells (ORSCs), has previously demonstrated pluripotency for differentiation into neural stem cells (NSCs). A previous study indicated that nestinpositive hair follicle ORSCs are able to differentiate into neurons. However, little has been reported on the isolation of nestinnegative human ORSCs and whether they can successfully differentiate into neurons in vitro. In the present study, nestinpositive ORSCs were significantly reduced with a prolonged incubation time in vitro. Following 9 days of primary culture, nestinexpressing ORSCs disappeared entirely, and ORSCs remained nestinnegative following 5 days of subculture. Notably, nestin was identified in ORSCs following a threestep process of neuroinduction. In addition, neruodevelopmental markers were detected in the ORSCderived nestinpositive spherical cell mass, including the induction of the neuronal specific markers growth associated protein43, neurotensin receptor3 and p75 neurotrophin receptor, and also the gliocyte markers, glial fibrillary acidic protein and S100. These sphereforming cells did not express the mature neuronassociated markers neurofilament medium, neuronal nuclei and neuronspecific enolase, which suggested that sphereforming cells may preferentially differentiate into neural stem celllike cells as opposed to mature neurons or neurogliocyte. In conclusion, ORSCdriven neural differentiation may be a suitable treatment strategy for neurodegenerative diseases and may possess an important value in regenerative medicine.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Células Madre
/
Diferenciación Celular
/
Folículo Piloso
/
Nestina
/
Neuronas
Límite:
Humans
/
Middle aged
Idioma:
En
Revista:
Mol Med Rep
Año:
2017
Tipo del documento:
Article