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A Semi-automated High-Throughput Microtitration Assay for Filoviruses.
Filone, Claire Marie; Miller, David; Wahl-Jensen, Victoria.
Afiliación
  • Filone CM; Department of Homeland Security, National Biological Threat Characterization Center, National Biodefense Analysis & Countermeasures Center, Science & Technology Directorate, Fort Detrick, MD, USA.
  • Miller D; Department of Homeland Security, National Biological Threat Characterization Center, National Biodefense Analysis & Countermeasures Center, Science & Technology Directorate, Fort Detrick, MD, USA.
  • Wahl-Jensen V; Department of Homeland Security, National Biological Threat Characterization Center, National Biodefense Analysis & Countermeasures Center, Science & Technology Directorate, Fort Detrick, MD, USA. victoria.jensen@nbacc.dhs.gov.
Methods Mol Biol ; 1628: 163-175, 2017.
Article en En | MEDLINE | ID: mdl-28573618
ABSTRACT
The 50% tissue culture infectious dose (TCID50) endpoint dilution assay is one of the gold standard methods for measuring filovirus infectivity. We have increased virology microtitration assay throughput at biosafety level (BSL)-4 by implementing automated liquid handling and semi-automated assay endpoint readout. Utilization of automated liquid handling for cell plating and virus dilution along with optimization of the assay endpoint readout, using a luminescent-based cell viability assay and an automated plate reader, has improved workflow efficiency, reduced operator burden and assay time, decreased assay variability, and increased data return.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Filoviridae / Ensayos Analíticos de Alto Rendimiento Límite: Humans Idioma: En Revista: Methods Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2017 Tipo del documento: Article País de afiliación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Filoviridae / Ensayos Analíticos de Alto Rendimiento Límite: Humans Idioma: En Revista: Methods Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2017 Tipo del documento: Article País de afiliación: Estados Unidos