Rapid preparation and single-cell analysis of concentrated blood smears using a high-throughput blood cell separator and a microfabricated grid film.
J Chromatogr A
; 1507: 141-148, 2017 Jul 21.
Article
en En
| MEDLINE
| ID: mdl-28576331
ABSTRACT
Cytological examination of peripheral white blood cells inhomogeneously distributed on a blood smear is currently limited by the low abundance and random sampling of the target cells. To address the challenges, we present a new approach to prepare and analyze concentrated blood smears by rapidly enriching white blood cells up to 32-fold with 92% recovery on average at a high throughput (1mL/min) using a deterministic migration-based separator and by systematically analyzing a large number of the cells distributed over a blood slide using a microfabricated grid film. We anticipate that our approach will improve the clinical utility of blood smear tests, while offering the capability to detect rare cell populations.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Células Sanguíneas
/
Separación Celular
/
Microtecnología
/
Análisis de la Célula Individual
Tipo de estudio:
Evaluation_studies
Límite:
Humans
Idioma:
En
Revista:
J Chromatogr A
Año:
2017
Tipo del documento:
Article