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Comparison of the XTT and resazurin assays for quantification of the metabolic activity of Staphylococcus aureus biofilm.
Alonso, Beatriz; Cruces, Raquel; Pérez, Adrián; Sánchez-Carrillo, Carlos; Guembe, María.
Afiliación
  • Alonso B; Department of Clinical Microbiology and Infectious Diseases, Hospital General Universitario Gregorio Marañón, Madrid, Spain; Instituto de Investigación Sanitaria Gregorio Marañón, Madrid, Spain.
  • Cruces R; Department of Clinical Microbiology and Infectious Diseases, Hospital General Universitario Gregorio Marañón, Madrid, Spain; Instituto de Investigación Sanitaria Gregorio Marañón, Madrid, Spain.
  • Pérez A; Biology Department, School of Biology, Universidad Autónoma de Madrid, Madrid, Spain.
  • Sánchez-Carrillo C; Department of Clinical Microbiology and Infectious Diseases, Hospital General Universitario Gregorio Marañón, Madrid, Spain.
  • Guembe M; Department of Clinical Microbiology and Infectious Diseases, Hospital General Universitario Gregorio Marañón, Madrid, Spain; Instituto de Investigación Sanitaria Gregorio Marañón, Madrid, Spain. Electronic address: maria.guembe@iisgm.es.
J Microbiol Methods ; 139: 135-137, 2017 08.
Article en En | MEDLINE | ID: mdl-28587857
ABSTRACT
We assessed whether resazurin was as efficient as XTT in the measurement of the metabolic activity of 209 clinical Staphylococcus aureus biofilm using an vitro model comparing the percentage of formazan and resorufin. The overall categorical agreement was 61.2% (r=0.024), which means that resazurin can not substitute XTT.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Oxazinas / Staphylococcus aureus / Sales de Tetrazolio / Xantenos Idioma: En Revista: J Microbiol Methods Año: 2017 Tipo del documento: Article País de afiliación: España
Texto completo
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Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Oxazinas / Staphylococcus aureus / Sales de Tetrazolio / Xantenos Idioma: En Revista: J Microbiol Methods Año: 2017 Tipo del documento: Article País de afiliación: España