Rad52 Inverse Strand Exchange Drives RNA-Templated DNA Double-Strand Break Repair.
Mol Cell
; 67(1): 19-29.e3, 2017 Jul 06.
Article
en En
| MEDLINE
| ID: mdl-28602639
ABSTRACT
RNA can serve as a template for DNA double-strand break repair in yeast cells, and Rad52, a member of the homologous recombination pathway, emerged as an important player in this process. However, the exact mechanism of how Rad52 contributes to RNA-dependent DSB repair remained unknown. Here, we report an unanticipated activity of yeast and human Rad52 inverse strand exchange, in which Rad52 forms a complex with dsDNA and promotes strand exchange with homologous ssRNA or ssDNA. We show that in eukaryotes, inverse strand exchange between homologous dsDNA and RNA is a distinctive activity of Rad52; neither Rad51 recombinase nor the yeast Rad52 paralog Rad59 has this activity. In accord with our in vitro results, our experiments in budding yeast provide evidence that Rad52 inverse strand exchange plays an important role in RNA-templated DSB repair in vivo.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Saccharomyces cerevisiae
/
Moldes Genéticos
/
ADN de Hongos
/
ADN de Cadena Simple
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ARN de Hongos
/
Proteínas de Saccharomyces cerevisiae
/
Reparación del ADN
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Proteína Recombinante y Reparadora de ADN Rad52
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Roturas del ADN de Doble Cadena
Límite:
Humans
Idioma:
En
Revista:
Mol Cell
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2017
Tipo del documento:
Article
País de afiliación:
Estados Unidos