Revisiting the genotyping scheme for varicella-zoster viruses based on whole-genome comparisons.
J Gen Virol
; 98(6): 1434-1438, 2017 06.
Article
en En
| MEDLINE
| ID: mdl-28613146
ABSTRACT
We report whole-genome sequences (WGSs) for four varicella-zoster virus (VZV) samples from a shingles study conducted by Kaiser Permanente of Southern California. Comparative genomics and phylogenetic analysis of all published VZV WGSs revealed that strain KY037798 is in clade IX, which shall henceforth be designated clade 9. Previously published single nucleotide polymorphisms (SNP)-based genotyping schemes fail to discriminate between clades 6 and VIII and employ positions that are not clade-specific. We provide an updated list of clade-specific positions that supersedes the list determined at the 2008 VZV nomenclature meeting. Finally, we propose a new targeted genotyping scheme that will discriminate the circulating VZV clades with at least a twofold redundancy. Genotyping strategies using a limited set of targeted SNPs will continue to provide an efficient 'first pass' method for VZV strain surveillance as vaccination programmes for varicella and zoster influence the dynamics of VZV transmission.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Filogenia
/
Variación Genética
/
Herpesvirus Humano 3
/
Genómica
/
Técnicas de Genotipaje
/
Genotipo
Límite:
Humans
País/Región como asunto:
America do norte
Idioma:
En
Revista:
J Gen Virol
Año:
2017
Tipo del documento:
Article
País de afiliación:
Estados Unidos