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Isolation and characterization of Sprague-Dawley and Wistar Kyoto GFP rat embryonic stem cells.
Coppiello, Giulia; Abizanda, Gloria; Aguado, Natalia; Iglesias, Elena; Iglesias-Garcia, Olalla; Lo Nigro, Antonio; Prosper, Felipe; Aranguren, Xabier L.
Afiliación
  • Coppiello G; Cell Therapy Program, Center for Applied Medical Research (CIMA), University of Navarra, Health Research Institute of Navarra, Pamplona, Spain.
  • Abizanda G; Cell Therapy Program, Center for Applied Medical Research (CIMA), University of Navarra, Health Research Institute of Navarra, Pamplona, Spain.
  • Aguado N; Cell Therapy Program, Center for Applied Medical Research (CIMA), University of Navarra, Health Research Institute of Navarra, Pamplona, Spain.
  • Iglesias E; Cell Therapy Program, Center for Applied Medical Research (CIMA), University of Navarra, Health Research Institute of Navarra, Pamplona, Spain.
  • Iglesias-Garcia O; Center of Regenerative Medicine in Barcelona (CMRB), Spain.
  • Lo Nigro A; Ri.Med Foundation, Department of Laboratory Medicine and Advanced Biotechnologies, IRCCS-ISMETT (Istituto Mediterraneo per i Trapianti e Terapie ad Alta Specializzazione), Palermo, Italy.
  • Prosper F; Cell Therapy Program, Center for Applied Medical Research (CIMA), University of Navarra, Health Research Institute of Navarra, Pamplona, Spain; Cell Therapy Area, Clínica Universidad de Navarra, University of Navarra, Health Research Institute of Navarra, Pamplona, Spain. Electronic address: fprospe
  • Aranguren XL; Cell Therapy Program, Center for Applied Medical Research (CIMA), University of Navarra, Health Research Institute of Navarra, Pamplona, Spain. Electronic address: xlaranguren@unav.es.
Stem Cell Res ; 21: 40-43, 2017 05.
Article en En | MEDLINE | ID: mdl-28677536
We generated two rat embryonic stem cell (ESC) lines: ATCe-SD7.8 from Sprague-Dawley strain and ATCe-WK1 from Wistar Kyoto strain. Cells were marked with enhanced green fluorescent protein (eGFP) by transduction with a lentiviral vector. Cells present a normal karyotype and express pluripotency-associated markers. Pluripotency was tested in vivo with the teratoma formation assay. Cells maintain eGFP expression upon differentiation to the three-germ layers. These cells can be a useful tool for cell therapy studies and chimera generation as they can be easily tracked by eGFP expression.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Separación Celular / Proteínas Fluorescentes Verdes / Células Madre Embrionarias Límite: Animals Idioma: En Revista: Stem Cell Res Año: 2017 Tipo del documento: Article País de afiliación: España Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Separación Celular / Proteínas Fluorescentes Verdes / Células Madre Embrionarias Límite: Animals Idioma: En Revista: Stem Cell Res Año: 2017 Tipo del documento: Article País de afiliación: España Pais de publicación: Reino Unido