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High-throughput analysis of TUNEL-stained sperm using image cytometry.
Arifulin, E A; Bragina, E E; Kurilo, L F; Sheval, E V.
Afiliación
  • Arifulin EA; A.N. Belozersky Institute of Physico-Chemical Biology, M.V. Lomonosov Moscow State University, Moscow, 119992, Russian Federation.
  • Bragina EE; Рeoples' Friendship University of Russia (RUDN University), 6 Miklukho-Maklaya St, Moscow, 117198, Russian Federation.
  • Kurilo LF; A.N. Belozersky Institute of Physico-Chemical Biology, M.V. Lomonosov Moscow State University, Moscow, 119992, Russian Federation.
  • Sheval EV; Federal State Budgetary Institution "Research Centre for Medical Genetics", Moscow, Russian Federation.
Cytometry A ; 91(9): 854-858, 2017 09.
Article en En | MEDLINE | ID: mdl-28678382
Sperm DNA integrity is an essential factor for successful fertilization and proper pregnancy progression. The terminal deoxynucleotidyl transferase dUTP nick end-labeling (TUNEL) assay is commonly used for the estimation of the DNA fragmentation index. Analysis of TUNEL-stained sperm is often performed by flow cytometry, an approach that allows high-throughput analysis but in which any morphological information is lost. In this study, results of an automated image cytometry estimation of TUNEL-stained sperms were presented. The results of visual counting and automatic analysis were closely correlated, indicating that image cytometry is suitable for such analysis and may be applied in a clinical setting. © 2017 International Society for Advancement of Cytometry.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Espermatozoides Límite: Female / Humans / Male / Pregnancy Idioma: En Revista: Cytometry A Año: 2017 Tipo del documento: Article Pais de publicación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Espermatozoides Límite: Female / Humans / Male / Pregnancy Idioma: En Revista: Cytometry A Año: 2017 Tipo del documento: Article Pais de publicación: Estados Unidos