High-throughput analysis of TUNEL-stained sperm using image cytometry.
Cytometry A
; 91(9): 854-858, 2017 09.
Article
en En
| MEDLINE
| ID: mdl-28678382
Sperm DNA integrity is an essential factor for successful fertilization and proper pregnancy progression. The terminal deoxynucleotidyl transferase dUTP nick end-labeling (TUNEL) assay is commonly used for the estimation of the DNA fragmentation index. Analysis of TUNEL-stained sperm is often performed by flow cytometry, an approach that allows high-throughput analysis but in which any morphological information is lost. In this study, results of an automated image cytometry estimation of TUNEL-stained sperms were presented. The results of visual counting and automatic analysis were closely correlated, indicating that image cytometry is suitable for such analysis and may be applied in a clinical setting. © 2017 International Society for Advancement of Cytometry.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Espermatozoides
Límite:
Female
/
Humans
/
Male
/
Pregnancy
Idioma:
En
Revista:
Cytometry A
Año:
2017
Tipo del documento:
Article
Pais de publicación:
Estados Unidos