Low-density lipoproteins investigated under high hydrostatic pressure by elastic incoherent neutron scattering.
Eur Phys J E Soft Matter
; 40(7): 68, 2017 Jul.
Article
en En
| MEDLINE
| ID: mdl-28733727
ABSTRACT
Human low-density lipoprotein (LDL) is a highly complex nano-particle built up of various lipid classes and a single large protein moiety (apoB-100) owning essential physiological functions in the human body. Besides its vital role as a supplier of cholesterol and fat for peripheral tissues and cells, it is also a known key player in the formation of atherosclerosis. Due to these important roles in physiology and pathology the elucidation of structural and dynamical details is of great interest. In the current study we drew a broader picture of LDL dynamics using elastic incoherent neutron scattering (EINS) as a function of specified temperature and pressure points. We not only investigated a normolipidemic LDL sample, but also a triglyceride-rich and an oxidized one to mimic pathologic conditions as found under hyperlipidemic conditions or in atherosclerotic plaques, respectively. We could show that pressure has a significant effect on atomic motions in modified forms of LDL, whereas the normolipidemic sample seems to cope much better with high-pressure conditions irrespective of temperature. These findings might be explained by the altered lipid composition, which is either caused through elevated triglyceride content or modifications through lipid peroxidation.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Difracción de Neutrones
/
Elasticidad
/
Lipoproteínas LDL
Límite:
Humans
Idioma:
En
Revista:
Eur Phys J E Soft Matter
Asunto de la revista:
BIOFISICA
Año:
2017
Tipo del documento:
Article
País de afiliación:
Francia