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Phenotype microarray analysis of the AdeRS two-component system in Acinetobacter baumannii.
Sun, J-R; Chiang, Y-S; Shang, H-S; Perng, C-L; Yang, Y-S; Chiueh, T-S.
Afiliación
  • Sun JR; Division of Clinical Pathology, Department of Pathology, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan.
  • Chiang YS; Graduate Institute of Pathology, National Defense Medical Center, Taipei, Taiwan.
  • Shang HS; Department of Laboratory Medicine, Huilai Medical Care Corporation Hongren Hospital, Yuanlin Township, Changhua County, Taiwan.
  • Perng CL; Division of Clinical Pathology, Department of Pathology, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan.
  • Yang YS; Division of Clinical Pathology, Department of Pathology, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan.
  • Chiueh TS; Graduate Institute of Pathology, National Defense Medical Center, Taipei, Taiwan.
Eur J Clin Microbiol Infect Dis ; 36(12): 2343-2353, 2017 Dec.
Article en En | MEDLINE | ID: mdl-28741095
ABSTRACT
Acinetobacter baumannii is a nosocomial pathogen capable of resistance to multiple antimicrobials. The AdeRS two-component system (TCS) is associated with antimicrobial resistance by controlling the AdeABC efflux pump. To elucidate modulation by AdeRS, we made an A. baumannii mutant lacking the AdeRS TCS and characterized it using phenotype microarray (PM) analysis. After disrupting the adeRS operon, lower expression of AdeABC efflux pump was observed in the mutant strain. PM analysis showed that the AdeRS deletion strain and parental strain presented different tolerances to 91 compounds. Tolerance to 54 of the 91 compounds could be restored by complementing the AdeRS deleted strain with a plasmid carrying the adeRS gene. Compared to the parental strain, the AdeRS deletion strain was more sensitive to various inhibitors that target on-protein synthesis and function of cell membrane permeability. Tolerance to phleomycin of the AdeRS deletion strain reduced greatly and was further confirmed with minimum inhibitory concentration (MIC) determination and spot assay. The efflux pump inhibitor, NMP, could reduce phleomycin MIC four-fold at least for 29 (34.8%) of 81 tigecycline-resistant extensively drug-resistant A. baumannii (TGC-resistant XDRAB) clinical isolates. Our results suggested that the AdeRS TCS of A. baumannii was important for both elimination of antibiotics and tolerance to particular compounds.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas de Transporte de Membrana / Fenotipo / Farmacorresistencia Bacteriana / Acinetobacter baumannii Límite: Humans Idioma: En Revista: Eur J Clin Microbiol Infect Dis Asunto de la revista: DOENCAS TRANSMISSIVEIS / MICROBIOLOGIA Año: 2017 Tipo del documento: Article País de afiliación: Taiwán

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas de Transporte de Membrana / Fenotipo / Farmacorresistencia Bacteriana / Acinetobacter baumannii Límite: Humans Idioma: En Revista: Eur J Clin Microbiol Infect Dis Asunto de la revista: DOENCAS TRANSMISSIVEIS / MICROBIOLOGIA Año: 2017 Tipo del documento: Article País de afiliación: Taiwán