Your browser doesn't support javascript.
loading
Ehrlich Reaction Evoked Multiple Spectral Resonances and Gold Nanoparticle Hotspots for Raman Detection of Plant Hormone.
Wang, Fangyuan; Gu, Xiaoling; Zheng, Chunchen; Dong, Fang; Zhang, Liying; Cai, Yueqing; You, Zhengyi; You, Junhui; Du, Shuhu; Zhang, Zhongping.
Afiliación
  • Wang F; School of Pharmacy, Nanjing Medical University , Nanjing, Jiangsu 211166, China.
  • Gu X; School of Pharmacy, Nanjing Medical University , Nanjing, Jiangsu 211166, China.
  • Zheng C; School of Pharmacy, Nanjing Medical University , Nanjing, Jiangsu 211166, China.
  • Dong F; School of Pharmacy, Nanjing Medical University , Nanjing, Jiangsu 211166, China.
  • Zhang L; School of Pharmacy, Nanjing Medical University , Nanjing, Jiangsu 211166, China.
  • Cai Y; School of Pharmacy, Nanjing Medical University , Nanjing, Jiangsu 211166, China.
  • You Z; School of Pharmacy, Nanjing Medical University , Nanjing, Jiangsu 211166, China.
  • You J; School of Pharmacy, Nanjing Medical University , Nanjing, Jiangsu 211166, China.
  • Du S; School of Pharmacy, Nanjing Medical University , Nanjing, Jiangsu 211166, China.
  • Zhang Z; School of Chemistry and Chemical Engineering, Anhui University , Hefei, Anhui 230601, China.
Anal Chem ; 89(17): 8836-8843, 2017 09 05.
Article en En | MEDLINE | ID: mdl-28745043
ABSTRACT
Surface-enhanced Raman scattering (SERS) by use of noble metal nanoparticles has become a powerful tool to determine a low-concentration target by unique spectral fingerprints, but it is still limited to the Raman-inactive and nonresonant biomolecules such as amine acids, proteins, and hormones. Here, we report an Ehrlich reaction based derivative strategy in combination with gold nanoparticles (Au NPs) hotspots for the selective detection of indole-like plant hormones by SERS spectroscopy. Ehrlich reaction of p-(dimethylamino)benzaldehyde (PDAB) with the indole ring chemically transformed plant hormone indole-3-butyric acid (IBA) into a Raman-active and resonant derivative with an extended π-conjugated system in the form of a cation, which produced a new absorption band at 626 nm. On the other hand, cationic IBA-PDAB highly evoked the aggregation of Au NPs with negative citrate ligands to form the effective Raman hotspots and gave rise to the new absorption ranging from 600 to 800 nm. Significantly, the spectral overlap among IBA-PDAB, aggregated Au NPs, and the exciting laser initiated the multiple optical resonances to generate the ultrahigh Raman scattering with a sensitive limit of 2.0 nM IBA. The IBA in the whole sprouts and various parts of pea, mungbean, soybean, and black bean has been identified and quantified. The reported method opens a novel avenue for the SERS detection of Raman-inactive analyte by a proper derivation.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Reguladores del Crecimiento de las Plantas / Espectrometría Raman / Nanopartículas del Metal / Oro Tipo de estudio: Diagnostic_studies Idioma: En Revista: Anal Chem Año: 2017 Tipo del documento: Article País de afiliación: China

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Reguladores del Crecimiento de las Plantas / Espectrometría Raman / Nanopartículas del Metal / Oro Tipo de estudio: Diagnostic_studies Idioma: En Revista: Anal Chem Año: 2017 Tipo del documento: Article País de afiliación: China