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Single Molecule Nanopore Spectrometry for Peptide Detection.
Chavis, Amy E; Brady, Kyle T; Hatmaker, Grace A; Angevine, Christopher E; Kothalawala, Nuwan; Dass, Amala; Robertson, Joseph W F; Reiner, Joseph E.
Afiliación
  • Chavis AE; Department of Physics, Virginia Commonwealth University , Richmond, Virginia 23284, United States.
  • Brady KT; Department of Physics, Virginia Commonwealth University , Richmond, Virginia 23284, United States.
  • Hatmaker GA; Department of Physics, Virginia Commonwealth University , Richmond, Virginia 23284, United States.
  • Angevine CE; Department of Physics, Virginia Commonwealth University , Richmond, Virginia 23284, United States.
  • Kothalawala N; Department of Chemistry and Biochemistry, University of Mississippi , University, Mississippi 38677, United States.
  • Dass A; Department of Chemistry and Biochemistry, University of Mississippi , University, Mississippi 38677, United States.
  • Robertson JWF; Physical Measurement Laboratory, National Institute of Standards and Technology , Gaithersburg, Maryland 20899-8120, United States.
  • Reiner JE; Department of Physics, Virginia Commonwealth University , Richmond, Virginia 23284, United States.
ACS Sens ; 2(9): 1319-1328, 2017 Sep 22.
Article en En | MEDLINE | ID: mdl-28812356
Sensing and characterization of water-soluble peptides is of critical importance in a wide variety of bioapplications. Single molecule nanopore spectrometry (SMNS) is based on the idea that one can use biological protein nanopores to resolve different sized molecules down to limits set by the blockade duration and noise. Previous work has shown that this enables discrimination between polyethylene glycol (PEG) molecules that differ by a single monomer unit. This paper describes efforts to extend SMNS to a variety of biologically relevant, water-soluble peptides. We describe the use of Au25(SG)18 clusters, previously shown to improve PEG detection, to increase the on- and off-rate of peptides to the pore. In addition, we study the role that fluctuations play in the single molecule nanopore spectrometry (SMNS) methodology and show that modifying solution conditions to increase peptide flexibility (via pH or chaotropic salt) leads to a nearly 2-fold reduction in the current blockade fluctuations and a corresponding narrowing of the peaks in the blockade distributions. Finally, a model is presented that connects the current blockade depths to the mass of the peptides, which shows that our enhanced SMNS detection improves the mass resolution of the nanopore sensor more than 2-fold for the largest cationic peptides studied.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Diagnostic_studies Idioma: En Revista: ACS Sens Año: 2017 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Diagnostic_studies Idioma: En Revista: ACS Sens Año: 2017 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos