Optimization of dextran production by Weissella cibaria NITCSK4 using Response Surface Methodology-Genetic Algorithm based technology.
Carbohydr Polym
; 174: 103-110, 2017 Oct 15.
Article
en En
| MEDLINE
| ID: mdl-28821024
ABSTRACT
The most influencing factor on dextran production by Weissella cibaria NITCSK4 were screened using Plackett Burman design at 95% confidence limit with higher value of co-efficient of determination (R2) 99.58%. The combined effects of significant factors, namely, sucrose, temperature, dipotassium hydrogen phosphate (K2HPO4) and yeast extract were studied and optimized using Response Surface Methodology (RSM). The input parameters of non-linear models predicted by RSM were subsequently optimized using the genetic algorithm (GA) for obtaining a maximum dextran yield. The maximum yield was obtained with sucrose concentration of 15.78%, yeast extract 1.27%, K2HPO4 1.25%, and at 26°C. The predicted conditions were experimentally validated and 43.79mg/ml of dextran was produced. The dextran yield was 51% higher as compared to unoptimized medium. The molecular weight of resulting dextran produced at 26°C is >2000kDa. The NMR spectroscopic analysis demonstrated that the NITCSK4 produced linear dextran with predominant α (1-6) linkage.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Microbiología Industrial
/
Dextranos
/
Weissella
Tipo de estudio:
Prognostic_studies
/
Risk_factors_studies
Idioma:
En
Revista:
Carbohydr Polym
Año:
2017
Tipo del documento:
Article
País de afiliación:
India