Method to minimize ozone effect on Cy5 fluorescent intensity in DNA microarrays.
Anal Biochem
; 538: 1-4, 2017 12 01.
Article
en En
| MEDLINE
| ID: mdl-28882748
ABSTRACT
Cyanine 5 (Cy5) is an established fluorescent dye in microarray analysis. It is degraded rapidly when exposed to atmospheric ozone during post-hybridization washes, which leads to loss of fluorescent intensity. To minimize this undesirable effect, we coated microarray slides with sodium dodecyl sulfate (SDS) solution at post-hybridization washes. The fluorescent intensities on coated slides were more stable than those on uncoated slide. We also performed the microarrays with SDS solution for a year to check the solution's effectiveness along with seasonal changes of atmospheric ozone level. Consistent results in microarray analysis were obtained using Cy5 dye under atmospheric ozone.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Ozono
/
ADN
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Carbocianinas
/
Análisis de Secuencia por Matrices de Oligonucleótidos
/
Colorantes Fluorescentes
Idioma:
En
Revista:
Anal Biochem
Año:
2017
Tipo del documento:
Article