Restoration of YAP activation rescues HL-1 cardiomyocytes from apoptotic death by ethanol.
J Toxicol Sci
; 42(5): 545-551, 2017.
Article
en En
| MEDLINE
| ID: mdl-28904289
ABSTRACT
We reported previously that when mouse atrium-derived HL-1 cardiomyocytes undergo apoptosis upon exposure to 2% ethanol, the cellular cytoskeleton is severely disrupted and the anti-apoptotic transcriptional co-activator Yes-associated protein (YAP) is inactivated. Consistent with our previous observations, the expression of connective tissue growth factor (CTGF), an anti-apoptotic growth factor and a target of YAP, decreases in a time-dependent manner during exposure to 2% ethanol. The restoration of YAP activation rescues the cells from apoptosis both the retrovirus-mediated expression of constitutively active YAP and the stabilization of the actomyosin cytoskeleton by jasplakinolide prevent cell death. In contrast, YAP inhibitors have no effect on cell death, confirming the inactivation of YAP in ethanol-exposed cells. Thus, a decrease in actin tension and YAP inactivation should be crucially involved in the cytotoxicity of ethanol on HL-1 cardiomyocytes.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Fosfoproteínas
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Apoptosis
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Miocitos Cardíacos
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Proteínas Adaptadoras Transductoras de Señales
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Etanol
Límite:
Animals
Idioma:
En
Revista:
J Toxicol Sci
Año:
2017
Tipo del documento:
Article