Determination of urea content in urea cream by centrifugal partition chromatography.
J Food Drug Anal
; 24(2): 399-405, 2016 04.
Article
en En
| MEDLINE
| ID: mdl-28911595
ABSTRACT
The objective of this study is to establish a centrifugal partition chromatography (CPC) method for determination of the urea ingredient in urea cream. The mechanism of this method is that urea is determined by UV detector at 430 nm after being extracted from the cream and derivatized on line via Ehrlich reaction in rotor of CPC, where the reaction products dissolve in the mobile phase and the cream matrix retains in the stationary phase. The mixed solvent consisting of n-hexane, methanol, hydrochloric acid and p-dimethylaminobenzaldehyde with a ratio of 1000 mL1000 mL18 mL2.0 g is used for solvent system of CPC. The CPC method proposed offers good precision and convenience without complex sample pretreatment processes.
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1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Urea
Idioma:
En
Revista:
J Food Drug Anal
Año:
2016
Tipo del documento:
Article