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A Rapid Blood Test To Determine the Active Status and Duration of Acute Viral Infection.
Zheng, Tianyu; Finn, Caroline; Parrett, Christopher J; Dhume, Kunal; Hwang, Ji Hae; Sidhom, David; Strutt, Tara M; Li Sip, Yuen Yee; McKinstry, Karl K; Huo, Qun.
Afiliación
  • Zheng T; Department of Chemistry and NanoScience Technology Center, University of Central Florida , 12424 Research Parkway Suite 400, Orlando, Florida 32826, United States.
  • Finn C; Burnett School of Biomedical Science, Division of Immunity and Pathogenesis, College of Medicine, University of Central Florida , 6900 Lake Nona Blvd., Orlando, Florida 32827, United States.
  • Parrett CJ; Burnett School of Biomedical Science, Division of Immunity and Pathogenesis, College of Medicine, University of Central Florida , 6900 Lake Nona Blvd., Orlando, Florida 32827, United States.
  • Dhume K; Burnett School of Biomedical Science, Division of Immunity and Pathogenesis, College of Medicine, University of Central Florida , 6900 Lake Nona Blvd., Orlando, Florida 32827, United States.
  • Hwang JH; Burnett School of Biomedical Science, Division of Immunity and Pathogenesis, College of Medicine, University of Central Florida , 6900 Lake Nona Blvd., Orlando, Florida 32827, United States.
  • Sidhom D; Burnett School of Biomedical Science, Division of Immunity and Pathogenesis, College of Medicine, University of Central Florida , 6900 Lake Nona Blvd., Orlando, Florida 32827, United States.
  • Strutt TM; Burnett School of Biomedical Science, Division of Immunity and Pathogenesis, College of Medicine, University of Central Florida , 6900 Lake Nona Blvd., Orlando, Florida 32827, United States.
  • Li Sip YY; Burnett School of Biomedical Science, Division of Immunity and Pathogenesis, College of Medicine, University of Central Florida , 6900 Lake Nona Blvd., Orlando, Florida 32827, United States.
  • McKinstry KK; Burnett School of Biomedical Science, Division of Immunity and Pathogenesis, College of Medicine, University of Central Florida , 6900 Lake Nona Blvd., Orlando, Florida 32827, United States.
  • Huo Q; Department of Chemistry and NanoScience Technology Center, University of Central Florida , 12424 Research Parkway Suite 400, Orlando, Florida 32826, United States.
ACS Infect Dis ; 3(11): 866-873, 2017 11 10.
Article en En | MEDLINE | ID: mdl-28918638
The ability to rapidly detect and diagnose acute viral infections is crucial for infectious disease control and management. Serology testing for the presence of virus-elicited antibodies in blood is one of the methods used commonly for clinical diagnosis of viral infections. However, standard serology-based tests have a significant limitation: they cannot easily distinguish active from past, historical infections. As a result, it is difficult to determine whether a patient is currently infected with a virus or not, and on an optimal course of action, based off of positive serology testing responses. Here, we report a nanoparticle-enabled blood test that can help overcome this major challenge. The new test is based on the analysis of virus-elicited immunoglobulin G (IgG) antibody present in the protein corona of a gold nanoparticle surface upon mixing the gold nanoparticles with blood sera. Studies conducted on mouse models of influenza A virus infection show that the test gives positive responses only in the presence of a recent acute viral infection, approximately between day 14 and day 21 following the infection, and becomes negative thereafter. When used together with the traditional serology testing, the nanoparticle test can determine clearly whether a positive serology response is due to a recent or historical viral infection. This new blood test can provide critical clinical information needed to optimize further treatment and/or to determine if further quarantining should be continued.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Pruebas Serológicas / Inmunohistoquímica / Infecciones por Orthomyxoviridae / Nanopartículas del Metal / Oro Tipo de estudio: Diagnostic_studies / Prognostic_studies Límite: Animals Idioma: En Revista: ACS Infect Dis Año: 2017 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Pruebas Serológicas / Inmunohistoquímica / Infecciones por Orthomyxoviridae / Nanopartículas del Metal / Oro Tipo de estudio: Diagnostic_studies / Prognostic_studies Límite: Animals Idioma: En Revista: ACS Infect Dis Año: 2017 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos