A ratiometric fluorescent pH probe based on keto-enol tautomerization for imaging of living cells in extreme acidity.
Analyst
; 142(20): 3906-3912, 2017 Oct 09.
Article
en En
| MEDLINE
| ID: mdl-28920604
ABSTRACT
6-(Diethylamino)-2,3-dihydro-1H-xanthene-4-carbaldehyde (DDXC), a reported synthetic intermediate for near-infrared fluorescent dyes, was developed into a fluorescent pH probe for extreme acidity. The unique sensing mechanism of DDXC for pH is based on the reversible protonation of the carbonyl oxygen followed by keto-enol tautomerization. The probe displays a linear ratiometric fluorescence response (I512/I580) to H+ over the extremely acidic range of pH 2.0-4.0 with a pKa of 3.11, and features high fluorescence quantum yield (Φ = 0.60) and excellent selectivity. More importantly, the probe can be applied to ratiometric fluorescence imaging of pH changes in living cells, making it a potential molecular tool for pH-related cell biology study.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Espectrometría de Fluorescencia
/
Colorantes Fluorescentes
/
Concentración de Iones de Hidrógeno
Límite:
Humans
Idioma:
En
Revista:
Analyst
Año:
2017
Tipo del documento:
Article