Expression of cross-tolerance to a wide range of conditions in a human lung cancer cell line after adaptation to nitric oxide.
Tumour Biol
; 39(9): 1010428317723778, 2017 Sep.
Article
en En
| MEDLINE
| ID: mdl-28936924
ABSTRACT
Previously, we have shown that A549, a human lung adenocarcinoma, can be adapted to nitric oxide (NOâ). NOâ is a nitrogen-based free radical that is synthesized by a family of enzymes known as nitric oxide synthases. NOâ has been shown to be overexpressed in patient populations of different cancers. In addition, it has been observed that patients who express high levels of nitric oxide synthases tend to have poorer clinical outcomes than those with low levels of expression. The original cell line A549 (parent) and the adapted A549-HNO (high nitric oxide) cell line serve as a useful model system to investigate the role of NOâ in tumor progression and prognosis. We have previously shown that the A549-HNO-adapted cells grow aggressively when compared to A549-parent cells. Furthermore, we have shown that the A549-HNO-adapted cells exhibit a higher percentage of cell viability when exposed to ultraviolet and X-ray radiation than the A549-parent cells. Cancer patients who develop resistance to one treatment often become resistant to other previously unencountered forms of treatment. This phenomenon is known as cross-tolerance. To determine whether NOâ is a potential cross-tolerance causing agent, we have expanded our research by conducting parallel studies to a variety of other agents and conditions beyond radiation and ultraviolet exposure. We exposed both cell lines to varying levels of chemotherapeutic drugs (taxol and doxorubicin), temperature, pH, calcium chloride, cadmium chloride, copper chloride, sodium chloride, ferrous chloride, and sodium-R-lipoic acid. Our results show that the A549-HNO cells exhibit greater viability than the A549-parent cells when exposed to each of the various conditions. Therefore, NOâ is one potential driving force that can make tumor cells exhibit cross-tolerance.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Adaptación Fisiológica
/
Adenocarcinoma
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Resistencia a Antineoplásicos
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Neoplasias Pulmonares
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Óxido Nítrico
Límite:
Humans
Idioma:
En
Revista:
Tumour Biol
Asunto de la revista:
NEOPLASIAS
Año:
2017
Tipo del documento:
Article
País de afiliación:
Estados Unidos