A Polymer/Peptide Complex-Based Sensor Array That Discriminates Bacteria in Urine.
Angew Chem Int Ed Engl
; 56(48): 15246-15251, 2017 11 27.
Article
en En
| MEDLINE
| ID: mdl-28960676
ABSTRACT
A negatively charged poly(para-phenyleneethynylene) (PPE) forms electrostatic complexes with four positively charged antimicrobial peptides (AMP). The AMPs partially quench the fluorescence of the PPE and discriminate fourteen different bacteria in water and in human urine by pattern-based fluorescence recognition; the AMP-PPE complexes bind differentially to the components of bacterial surfaces. The bacterial species and strains form clusters according to staining properties (Gram-positive and Gram-negative) or genetic similarity (genus, species, and strain). The identification and data treatment is performed by pattern evaluation with linear discriminant analysis (LDA) of the collected fluorescence intensity data.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Orina
/
Péptidos Catiónicos Antimicrobianos
/
Alquinos
/
Escherichia coli
/
Éteres
/
Micrococcaceae
Tipo de estudio:
Prognostic_studies
Límite:
Humans
Idioma:
En
Revista:
Angew Chem Int Ed Engl
Año:
2017
Tipo del documento:
Article
País de afiliación:
Alemania