Involvement of the JAK-STAT pathway in collagen regulation of decidual NK cells.
Am J Reprod Immunol
; 78(6)2017 Dec.
Article
en En
| MEDLINE
| ID: mdl-28975683
ABSTRACT
PROBLEM:
The mechanisms underlying the regulation of decidual natural killer cells (dNKs) at the maternal-fetal interface are unclear. METHOD OF STUDY Primary trophoblasts (TROs), decidual stromal cells (DSCs), and dNKs were cocultured, and responses to LAIR-2 (LAIR-1 inhibitor) and P4H shRNA (collagen inhibitor) were studied.RESULTS:
Coculture of dNKs with primary TROs/DSCs resulted in downregulation of Th1 cytokine production by dNKs. These effects were abrogated by LAIR-2 and P4H shRNA. LAIR-1 binds to SHP-1, which in turn binds to JAK1 and JAK2. Further, the phosphorylation of STAT1/STAT4 and the expression of the downstream transcription factors T-bet and Helios in dNKs were decreased by collagen treatment and primary TROs/DSCs coculture.CONCLUSION:
The JAK-STAT pathway and its downstream transcription factors T-bet and Helios are involved in the regulation of dNK function by collagen/LAIR-1 interaction, and this signaling mechanism may contribute to the maintenance of immune tolerance at the maternal-fetal interface.Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Trofoblastos
/
Células Asesinas Naturales
/
Colágeno
/
Células del Estroma
/
Decidua
Límite:
Female
/
Humans
/
Pregnancy
Idioma:
En
Revista:
Am J Reprod Immunol
Año:
2017
Tipo del documento:
Article
País de afiliación:
China