Determination of Total Biotin by Liquid Chromatography Coupled with Immunoaffinity Column Cleanup Extraction: Multilaboratory Testing, Final Action 2016.02.
J AOAC Int
; 101(3): 831-842, 2018 May 01.
Article
en En
| MEDLINE
| ID: mdl-29021006
ABSTRACT
Single- and multilaboratory testing data have provided systematic scientific evidence that a simple, selective, accurate, and precise method can be used as a potential candidate reference method for dispute resolution in determining total biotin in all forms of infant, adult, and/or pediatric formula. Using LC coupled with immunoaffinity column cleanup extraction, the method fully meets the intended purpose and applicability statement in AOAC Standard Method Performance Requirement 2014.005. The method was applied to a cross-section of infant formula and adult nutritional matrixes, and acceptable precision and accuracy were established. The analytical platform is inexpensive, and the method can be used in almost any laboratory worldwide with basic facilities. The immunoaffinity column cleanup extraction is the key step to successful analysis.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Biotina
/
Cromatografía de Afinidad
/
Cromatografía Liquida
/
Fórmulas Infantiles
Tipo de estudio:
Clinical_trials
Límite:
Adult
/
Child, preschool
/
Humans
/
Infant
/
Newborn
Idioma:
En
Revista:
J AOAC Int
Año:
2018
Tipo del documento:
Article
País de afiliación:
Nueva Zelanda