Co-immunoprecipitation for Identifying Protein-Protein Interactions in Borrelia burgdorferi.
Methods Mol Biol
; 1690: 47-55, 2018.
Article
en En
| MEDLINE
| ID: mdl-29032535
ABSTRACT
Co-immunoprecipitation can be utilized to study protein-protein interactions from various environments, cell types, or tissues. Herein, we describe a co-immunoprecipitation protocol that can be used to examine protein complexes found in the pathogenic spirochete Borrelia burgdorferi. The method outlined here has successfully identified known and unknown members of borrelial protein complexes and is an efficient method for studying protein interactions in this pathogenic spirochete.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Proteínas Bacterianas
/
Borrelia burgdorferi
/
Mapeo de Interacción de Proteínas
/
Inmunoprecipitación
/
Mapas de Interacción de Proteínas
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Methods Mol Biol
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2018
Tipo del documento:
Article
País de afiliación:
Estados Unidos