The Antimicrobial Peptide LL-37 as a Possible Adjunct for the Proliferation and Differentiation of Dental Pulp Stem Cells.
J Endod
; 43(12): 2048-2053, 2017 Dec.
Article
en En
| MEDLINE
| ID: mdl-29033090
ABSTRACT
INTRODUCTION:
This study evaluated the biocompatibility of 5 and 10 µg/mL LL-37 in vitro and its effect on the differentiation of human dental pulp stem cells (DPSCs) into odontoblast-like cells.METHODS:
Cell viability, genotoxicity, nitric oxide production, cell cycle, dentine sialophosphoprotein (DSPP) production, and DSPP gene expression.RESULTS:
Concentrations of 5 and 10 µg/mL of LL-37 were not cytotoxic and generally increased cell viability, especially on the third day (P < .05). The tested concentrations did not induce genotoxicity (P < .05). LL-37 did not significantly alter nitrite production at either concentration. Cell cycle analysis revealed that 10 µg/mL of LL-37 arrested cells in G0/G1 (P < .05). The control group exhibited higher numbers of cells in other phases of the cell cycle (P < .05). The expression of the DSPP protein and gene was also higher in the 10 µg/mL of LL-37 group (P < .05).CONCLUSIONS:
These results demonstrated that LL-37 was biocompatible at these concentrations and increased the number of viable cells, especially during the initial period. The 10 µg/mL concentration arrested the cell cycle and increased expression of the DSPP protein and gene, which indicates that this peptide contributes to odontoblastic differentiation.Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Células Madre
/
Diferenciación Celular
/
Pulpa Dental
/
Proliferación Celular
/
Catelicidinas
Límite:
Humans
Idioma:
En
Revista:
J Endod
Año:
2017
Tipo del documento:
Article
País de afiliación:
Brasil